<i><i>Acanthamoeba</i> polyphaga</i> strain – causative agent of vision-threatening keratitis uncommonly detected in Poland, susceptible in vitro to toyocamycin

Marcin Padzik; Jacek P. Szaflik; Justyna Izdebska; Beata Szostakowska; Jerzy Szaflik; Lidia Chomicz

doi:10.5114/ko.2017.71774

POLSKI

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ISSN: 0023-2157

Klinika Oczna / Acta Ophthalmologica Polonica

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2/2017
vol. 119

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abstract:

Original paper

Acanthamoeba polyphaga strain – causative agent of vision-threatening keratitis uncommonly detected in Poland, susceptible in vitro to toyocamycin

Marcin Padzik

¹

,

Jacek P. Szaflik

^{2, 3}

,

Justyna Izdebska

^{2, 3}

,

Beata Szostakowska

⁴

,

Jerzy Szaflik

^{2, 3}

,

Lidia Chomicz

¹

Department of Medical Biology, Medical University of Warsaw, Warsaw, Poland
Department of Ophthalmology, Medical University of Warsaw, Warsaw, Poland
SPKSO Ophthalmic University Hospital, Warsaw, Poland
Department of Tropical Parasitology, Institute of Maritime and Tropical Medicine, Medical University of Gdańsk, Gdynia, Poland

DOI: https://doi.org/10.5114/ko.2017.71774

Online publish date: 2017/11/29

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AMA

Padzik M, Szaflik J, Izdebska J, Szostakowska B, Szaflik J, Chomicz L. Acanthamoeba polyphaga strain – causative agent of vision-threatening keratitis uncommonly detected in Poland, susceptible in vitro to toyocamycin. Klinika Oczna / Acta Ophthalmologica Polonica. 2017;119(2):77-82. doi:10.5114/ko.2017.71774.

APA

Padzik, M., Szaflik, J., Izdebska, J., Szostakowska, B., Szaflik, J.,  & Chomicz, L. (2017). Acanthamoeba polyphaga strain – causative agent of vision-threatening keratitis uncommonly detected in Poland, susceptible in vitro to toyocamycin. Klinika Oczna / Acta Ophthalmologica Polonica, 119(2), 77-82. https://doi.org/10.5114/ko.2017.71774

Chicago

Padzik, Marcin, Jacek P. Szaflik, Justyna Izdebska, Beata Szostakowska, Jerzy Szaflik,  and Lidia Chomicz. 2017. "Acanthamoeba polyphaga strain – causative agent of vision-threatening keratitis uncommonly detected in Poland, susceptible in vitro to toyocamycin". Klinika Oczna / Acta Ophthalmologica Polonica 119 (2): 77-82. doi:10.5114/ko.2017.71774.

Harvard

Padzik, M., Szaflik, J., Izdebska, J., Szostakowska, B., Szaflik, J.,  and Chomicz, L. (2017). Acanthamoeba polyphaga strain – causative agent of vision-threatening keratitis uncommonly detected in Poland, susceptible in vitro to toyocamycin. Klinika Oczna / Acta Ophthalmologica Polonica, 119(2), pp.77-82. https://doi.org/10.5114/ko.2017.71774

MLA

Padzik, Marcin et al. "Acanthamoeba polyphaga strain – causative agent of vision-threatening keratitis uncommonly detected in Poland, susceptible in vitro to toyocamycin." Klinika Oczna / Acta Ophthalmologica Polonica, vol. 119, no. 2, 2017, pp. 77-82. doi:10.5114/ko.2017.71774.

Vancouver

Padzik M, Szaflik J, Izdebska J, Szostakowska B, Szaflik J, Chomicz L. Acanthamoeba polyphaga strain – causative agent of vision-threatening keratitis uncommonly detected in Poland, susceptible in vitro to toyocamycin. Klinika Oczna / Acta Ophthalmologica Polonica. 2017;119(2):77-82. doi:10.5114/ko.2017.71774.

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Introduction
Acanthamoebic keratitis, detected with increasing frequency worldwide in the last decades, mainly is reported in contact lens wearers. Other risk factors include damage to corneal cells, eye surgery and ocular exposure to water containing developmental forms of the amphizoic amoebae. In this study we analyzed Acanthamoeba isolates from complicated keratitis and evaluated some corneal strains in terms of their in vitro susceptibility to toyocamycin.

Material and methods
Six strains of Acanthamoebic keratitis in contact lens wearers, one with a history of swimming in a lake, previously improperly diagnosed and treated were analyzed. Acanthamoeba corneal isolates of T4 genotype cultured in vitro were monitored. A. polyphaga corneal strain and environmental A. castellanii Neff exposed to toyocamycin, the agent with an established anti-trichomonal and anti-toxoplasmal activity, were evaluated.

Results
A. polyphaga strain, which is not commonly diagnosed as an etiological agent of Acanthamoeba keratitis in Poland, was the most virulent in vitro. Amoebostatic effect of toyocamycin, without stimulation of encystation, was stronger for the corneal strain than for the environmental A. castellanii Neff.

Conclusions
There is no sufficient knowledge of the epidemiology of Acanthamoeba keratitis in Poland. Extremely high antimicrobial resistance of Acanthamoeba cysts leads to treatment failure, thus chemicals of possible anti-amoebic activity are still tested. Promising results of our study of adenosine analogue as the agent against Acanthamoebapathogenic strains justifies further tests with this drug and various amphizoic amoebic strains. This was the first study of in vitro susceptibility of corneal Acanthamoeba strain to toyocamycin.

keywords:

Acanthamoeba polyphaga strain – causative agent of vision-threatening keratitis uncommonly detected in Poland, susceptible in vitro to toyocamycin

Marcin Padzik 1 , Jacek P. Szaflik 2, 3 , Justyna Izdebska 2, 3 , Beata Szostakowska 4 , Jerzy Szaflik 2, 3 , Lidia Chomicz 1

Acanthamoeba keratitis, A polyphaga corneal strain, in vitro monitoring, amoebostatic activity of toyocamycin

Marcin Padzik

¹

,

Jacek P. Szaflik

^{2, 3}

,

Justyna Izdebska

^{2, 3}

,

Beata Szostakowska

⁴

,

Jerzy Szaflik

^{2, 3}

,

Lidia Chomicz

¹