<i>Experimental immunology</i><br>The influence of the “bio-coat’s” type of culture dishes on the primary cultures of lung cancer cells

Justyna Woś; Monika Pieczykolan; Paweł Rybojad; Andrzej Jabłonka; Karolina Olszewska-Bożek; Jacek Tabarkiewicz

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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2/2011
vol. 36

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abstract:

Experimental immunology
The influence of the “bio-coat’s” type of culture dishes on the primary cultures of lung cancer cells

Justyna Woś

,

Monika Pieczykolan

,

Paweł Rybojad

,

Andrzej Jabłonka

,

Karolina Olszewska-Bożek

,

Jacek Tabarkiewicz

(Centr Eur J Immunol 2011; 36 (2): 70-75)

Online publish date: 2011/06/20

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Lung cancer has the highest mortality rate among both women and men all over the world. A more complete understanding of the molecular pathogenesis and biology of human lung carcinomas may contribute to designing more effective therapies in the future. This paper focuses on primary cultures of human lung cancer cells and their application as models of malignant biological behavior.

The aim of the study was to evaluate the growth of lung cancer cells on various culture plates and to choose the most optimal and efficient surfaces.

Tissues from 6 lung cancer patients were included in the study. Cells were cultured on plates coated with different kinds of surfaces (polylysine, fibronectin, untreated glass and borosilicate glass). The number and viability of obtained cells were counted.

Lung cancer cells grew differently on polylysine, fibronectin and glass surfaces. The results of this experiment suggest that the best surface for the growth of lung cancer cells is polylysine surface (CC2). CC2 surfaces gave the greatest increase in cells count among four examined surfaces. The lung cancer cells cultured on the surfaces made of borosilicate glass did not proliferate, therefore these kinds of dishes are rather not suitable for the primary culture of lung cancer cells.

keywords:

Experimental immunologyThe influence of the “bio-coat’s” type of culture dishes on the primary cultures of lung cancer cells

Justyna Woś , Monika Pieczykolan , Paweł Rybojad , Andrzej Jabłonka , Karolina Olszewska-Bożek , Jacek Tabarkiewicz

lung cancer, cell culture, growth factors, culture surfaces

Experimental immunology
The influence of the “bio-coat’s” type of culture dishes on the primary cultures of lung cancer cells

Justyna Woś

,

Monika Pieczykolan

,

Paweł Rybojad

,

Andrzej Jabłonka

,

Karolina Olszewska-Bożek

,

Jacek Tabarkiewicz