Experimental immunology 

Application of a flow cytometry in differentiation of antibodies accompanying platelets in Ehrlichia canis and Borrelia burgdorferi infections in dogs

Katarzyna Płoneczka-Janeczko; Krzysztof Rypuła

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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3/2011
vol. 36

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abstract:

Experimental immunology
Application of a flow cytometry in differentiation of antibodies accompanying platelets in Ehrlichia canis and Borrelia burgdorferi infections in dogs

Katarzyna Płoneczka-Janeczko

,

Krzysztof Rypuła

(Centr Eur J Immunol 2011; 36 (3): 174-179)

Online publish date: 2011/10/18

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Sera of dogs (n = 30), with serologically confirmed monocytic ehrlichiosis (Ehrlichia canis, n = 21) or borreliosis (Borrelia burgdorferi, n = 9) were tested for presence of antibodies accompanying platelets (anti-platelet antibodies – APA). The analysis was carried out in the flow cytometer based on the principle of indirect anti-globulin test, using FITC-labeled antiglobulin conjugate against canine IgG. Out of the 30 examined dogs, antibodies directed against thrombocytes were indicated in total 10 cases (33.3%).

keywords:

Experimental immunology Application of a flow cytometry in differentiation of antibodies accompanying platelets in Ehrlichia canis and Borrelia burgdorferi infections in dogs

Katarzyna Płoneczka-Janeczko , Krzysztof Rypuła

flow cytometry, antiplatelet antibodies, Ehrlichia canis, Borrelia burgdorferi

Experimental immunology
Application of a flow cytometry in differentiation of antibodies accompanying platelets in Ehrlichia canis and Borrelia burgdorferi infections in dogs

Katarzyna Płoneczka-Janeczko

,

Krzysztof Rypuła