Experimental immunology 
The effect of sterilization by irradiation of human pericardium and skin frozen tissues on their ability to influence the proliferation of endothelial cells in vitro

Robert Zdanowski; Wanda Stankiewicz; Artur Kamiński; Tomasz Grzela; Piotr Skopiński; Sławomir Lewicki; Ewa Skopińska-Różewska

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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2/2012
vol. 37

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abstract:

Experimental immunology
The effect of sterilization by irradiation of human pericardium and skin frozen tissues on their ability to influence the proliferation of endothelial cells in vitro

Robert Zdanowski

,

Wanda Stankiewicz

,

Artur Kamiński

,

Tomasz Grzela

,

Piotr Skopiński

,

Sławomir Lewicki

,

Ewa Skopińska-Różewska

(Centr Eur J Immunol 2012; 37 (2): 119-122)

Online publish date: 2012/05/22

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AMA

Zdanowski R, Stankiewicz W, Kamiński A, et al. Experimental immunology 
The effect of sterilization by irradiation of human pericardium and skin frozen tissues on their ability to influence the proliferation of endothelial cells in vitro. Central European Journal of Immunology. 2012;37(2):119-122.

APA

Zdanowski, R., Stankiewicz, W., Kamiński, A., Grzela, T., Skopiński, P.,  & Lewicki, S. et al. (2012). Experimental immunology 
The effect of sterilization by irradiation of human pericardium and skin frozen tissues on their ability to influence the proliferation of endothelial cells in vitro. Central European Journal of Immunology, 37(2), 119-122.

Chicago

Zdanowski, Robert, Wanda Stankiewicz, Artur Kamiński, Tomasz Grzela, Piotr Skopiński, Sławomir Lewicki,  and Ewa Skopińska-Różewska. 2012. "Experimental immunology 
The effect of sterilization by irradiation of human pericardium and skin frozen tissues on their ability to influence the proliferation of endothelial cells in vitro". Central European Journal of Immunology 37 (2): 119-122.

Harvard

Zdanowski, R., Stankiewicz, W., Kamiński, A., Grzela, T., Skopiński, P., Lewicki, S.,  and Skopińska-Różewska, E. (2012). Experimental immunology 
The effect of sterilization by irradiation of human pericardium and skin frozen tissues on their ability to influence the proliferation of endothelial cells in vitro. Central European Journal of Immunology, 37(2), pp.119-122.

MLA

Zdanowski, Robert et al. "Experimental immunology 
The effect of sterilization by irradiation of human pericardium and skin frozen tissues on their ability to influence the proliferation of endothelial cells in vitro." Central European Journal of Immunology, vol. 37, no. 2, 2012, pp. 119-122.

Vancouver

Zdanowski R, Stankiewicz W, Kamiński A, Grzela T, Skopiński P, Lewicki S et al. Experimental immunology 
The effect of sterilization by irradiation of human pericardium and skin frozen tissues on their ability to influence the proliferation of endothelial cells in vitro. Central European Journal of Immunology. 2012;37(2):119-122.

The delayed wound healing is considered as important medical, social and economical problem. The effective wound management is one of major goals in health system strategy. Various dressing materials were used for this purpose among them skin and pericardium. The aim of the present paper was to compare the effect of these two materials, frozen and radiation-sterilized or non-sterilized, on the proliferation of HECa10 endothelial cells (EC) in vitro.

The influence of tissue samples on EC proliferation was evaluated with the Alamar Blue (AB) reagent, allowing the evaluation of viability and metabolic activity of cells.

Stimulation of EC proliferation was obtained for all used materials except sterilized pericardium, in comparison to the control cultures containing 10% of FBS (fetal bovine serum). No difference was observed between sterilized and non-sterilized skin.

keywords:

Experimental immunology The effect of sterilization by irradiation of human pericardium and skin frozen tissues on their ability to influence the proliferation of endothelial cells in vitro

Robert Zdanowski , Wanda Stankiewicz , Artur Kamiński , Tomasz Grzela , Piotr Skopiński , Sławomir Lewicki , Ewa Skopińska-Różewska

biological dressings, pericardium, skin, endothelial cells, proliferation

Experimental immunology
The effect of sterilization by irradiation of human pericardium and skin frozen tissues on their ability to influence the proliferation of endothelial cells in vitro

Robert Zdanowski

,

Wanda Stankiewicz

,

Artur Kamiński

,

Tomasz Grzela

,

Piotr Skopiński

,

Sławomir Lewicki

,

Ewa Skopińska-Różewska