<i>Experimental immunology</i><br>
Toxicity of silver nanoparticles in monocytes and keratinocytes: potential to induce inflammatory reactions

Piotr Orłowski; Małgorzata Krzyżowska; Anna Winnicka; André Chwalibóg; Ewa Sawosz

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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2/2012
vol. 37

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abstract:

Experimental immunology
Toxicity of silver nanoparticles in monocytes and keratinocytes: potential to induce inflammatory reactions

Piotr Orłowski

,

Małgorzata Krzyżowska

,

Anna Winnicka

,

André Chwalibóg

,

Ewa Sawosz

(Centr Eur J Immunol 2012; 37 (2): 123-130)

Online publish date: 2012/05/22

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Silver nanoparticles are of interest to be used as antimicrobial agents in wound dressings and coatings in medical devices, but potential adverse effects have been reported in the literature. The possible local inflammatory response to silver nanoparticles and the role of cell death in determining these effects are largely unknown. Effects of the mixture of silver nanoparticles of different sizes were compared in in vitro assays for cytotoxicity, caspase-1 and caspase-9 activity and bax expression. In all tested concentrations, silver nanoparticles were more toxic for RAW 264.7 monocytes than for 291.03C keratinocytes and induced significant caspase-1 activity and necrotic cell death. In keratinocytes, more significantly than in macrophages, silver nanoparticles led to increase of caspase-9 activity and apoptosis. These results indicate that effects of silver nanoparticles depend on the type of exposed cells. In addition, the potency of silver nanoparticles to induce necrosis and caspase-1 activity in monocytes indicates their possible immunotoxic inflammatory potential.

keywords:

Experimental immunology Toxicity of silver nanoparticles in monocytes and keratinocytes: potential to induce inflammatory reactions

Piotr Orłowski , Małgorzata Krzyżowska , Anna Winnicka , André Chwalibóg , Ewa Sawosz

silver nanoparticles, toxicity, monocytes, keratinocytes, apoptosis

Experimental immunology
Toxicity of silver nanoparticles in monocytes and keratinocytes: potential to induce inflammatory reactions

Piotr Orłowski

,

Małgorzata Krzyżowska

,

Anna Winnicka

,

André Chwalibóg

,

Ewa Sawosz