<i>Staphylococcus enterotoxin </i>B-induced T cells can efficaciously protect against type 1 diabetes in non-obese diabetic mice

Yelei Guo; Lei Xia; Shilun Zhang; Jiang Zhong; Yu Chen

doi:10.5114/ceji.2015.54589

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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3/2015
vol. 40

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abstract:

Experimental immunology

Staphylococcus enterotoxin B-induced T cells can efficaciously protect against type 1 diabetes in non-obese diabetic mice

Yelei Guo

,

Lei Xia

,

Shilun Zhang

,

Jiang Zhong

,

Yu Chen

(Cent Eur J Immunol 2015; 40 (3): 292-299)

DOI: https://doi.org/10.5114/ceji.2015.54589

Online publish date: 2015/10/15

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Type 1 diabetes (T1D), an autoimmune disease, can be protected against by natural killer T (NKT) cells. Several attempts demonstrate that NKT cells also can be produced by inducing with Staphylococcus enterotoxin B (SEB) in addition to its classical activated antigen -galactosylceramide. Here, we examined a potential usage of SEB-induced T (SEB-T) cells for the treatment of T1D. We established the immunophenotypes of SEB-T cells via flow cytometry, and in consequence, enriched in CD8+NKT cells after SEB stimulated. A high level of transforming growth factor β (TGF-β), detected by RT-PCR and ELISA, was first observed to be expressed and secreted by these SEB-T cells. Mixed lymphocyte reactions indicated that SEB-T cells could not produce a response to mitogens and allogeneic lymphocyte, and can inhibit lymphocytes response to mitogens. In an animal model, our data indicated that infusion of SEB-T cells in non-obese diabetic mice was well tolerated and could ameliorate hyperglycemia and maintain the blood glucose nearly on normal level until sacrifice. Strikingly, infusion of SEB-T cells resulted in an increase in the serum TGF-βlevel. These data raise the possibility that SEB-T cells can protect against T1D, which is associated with NKT cells generated in these SEB-induced cells.

keywords:

Staphylococcus enterotoxin B-induced T cells can efficaciously protect against type 1 diabetes in non-obese diabetic mice

Yelei Guo , Lei Xia , Shilun Zhang , Jiang Zhong , Yu Chen

immunomodulatory, type 1 diabetes, Staphylococcus enterotoxin B, CD8+ natural killer T, transforming growth factor-β, adoptive transfer

Yelei Guo

,

Lei Xia

,

Shilun Zhang

,

Jiang Zhong

,

Yu Chen