ANRIL regulates retinoblastoma progression via targeting autophagy by miR-328-3p/TSC1/ULK signaling

Yang Yang; Yuezhi Zhang; Yanmei Fu; Shuanglian Li; Xiaolong Yin

doi:10.5114/pjp.2024.142177

eISSN: 2084-9869
ISSN: 1233-9687

Polish Journal of Pathology

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3/2024
vol. 75

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abstract:

Original paper

ANRIL regulates retinoblastoma progression via targeting autophagy by miR-328-3p/TSC1/ULK signaling

Yang Yang

¹

,

Yuezhi Zhang

¹

,

Yanmei Fu

¹

,

Shuanglian Li

¹

,

Xiaolong Yin

¹

Department of Ophthalmic Center, The Second Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, China

Pol J Pathol 2024; 75 (3): 228-235

DOI: https://doi.org/10.5114/pjp.2024.142177

Online publish date: 2024/08/12

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Retinoblastoma is the most common primary intraocular malignancy of childhood. The aim of our study was to investigate the role and regulatory mechanism of the long non-coding RNA ANRIL in retinoblastoma.

Here, our data demonstrated that ANRIL overexpression inhibited miR-328-3p expression, but promoted expression of autophagy-related proteins (LC3B, ATG5, and BECN1). Then we predicted the binding sites for ANRIL with miR-328-3p, and for miR-328 3p with TSC1/ULK2 3′-UTR, and confirmed the combination of miR-328-3p and ANRIL and TSC1/ULK2 3′-UTR. Importantly, the data showed that ANRIL overexpression promoted TSC1 and ULK2 expression, and inhibited the phosphorylation of mTOR. Finally, our results indicated that ANRIL overexpression facilitated Y79 cell proliferation and cisplatin-induced apoptosis.

Our results indicated that ANRIL promoted the proliferation and cisplatin resistance of Y79 cells through activating autophagy by promoting TSC1/ULK2 ex- pression via acting as a miR-328-3p sponge.

keywords:

ANRIL regulates retinoblastoma progression via targeting autophagy by miR-328-3p/TSC1/ULK signaling

Yang Yang 1 , Yuezhi Zhang 1 , Yanmei Fu 1 , Shuanglian Li 1 , Xiaolong Yin 1

retinoblastoma, competing endogenous RNA, long non-coding RNA, cisplatin resistance, proliferation

Yang Yang

¹

,

Yuezhi Zhang

¹

,

Yanmei Fu

¹

,

Shuanglian Li

¹

,

Xiaolong Yin

¹