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ISSN: 1899-1874
Medical Studies/Studia Medyczne
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1/2023
vol. 39
 
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abstract:
Original paper

Activities of antioxidant enzymes and concentrations of selected oxidative stress biomarkers in synovial membranes of patients diagnosed with osteoarthritis

Agnieszka Jakubowska
1
,
Michał Dobrakowski
1
,
Alina Ostałowska
1
,
Aleksandra Kasperczyk
1
,
Beata Hapeta-Zeman
1
,
Jakub Jakubowski
1
,
Bogdan Koczy
2
,
Tomasz Stołtny
2
,
Sławomir Kasperczyk
1

  1. Department of Biochemistry, Medical University of Silesia, Zabrze, Poland Head of the Department: Prof. Jolanta Zalejska-Fiolka PhD
  2. Department of Orthopedics, District Orthopaedic Hospital, Piekary Śląskie, Poland Head of the Department: Tomasz Stołtny MD, PhD
Medical Studies/Studia Medyczne 2023; 39 (1): 45–50
DOI: https://doi.org/10.5114/ms.2023.126294
Online publish date: 2023/03/31
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Introduction
Osteoarthritis is a chronic degenerative process of multifactor etiology, still largely unexplained. The activity of antioxidant enzymes and concentration of lipid peroxidation products in synovial membrane can be indirectly inferred from its oxidation-reduction status. The role of synovial membrane in the pathogenesis of osteoarthritis is relatively poorly understood, probably due to the difficulties in the acquisition of the material.

Aim of the research
We sought to understand the association between osteoarthritis development and the activities of several antioxidant enzymes and levels of selected oxidative stress biomarkers in synovial membranes of osteoarthritic patients.

Material and methods
The synovial tissue samples were collected from 67 patients. Of these patients, 48 underwent endoprosthetic total hip replacement due to osteoarthritis (the examined group), while 19 underwent surgical repair of a traumatic femoral neck fracture (the control group). Homogenates of synovial membrane were prepared according to our published method. The biochemical analysis included: activity of copper-zinc superoxide dismutase (CuZn-SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT), concentrations of protein, malondialdehyde (MDA), thiol groups, and total oxidant status (TOS).

Results
When comparing the examined group with the control group, the activity of CuZn-SOD was significantly elevated and the activity of GPx and the level of thiol groups were significantly decreased, whereas the activities of GR, CAT, the levels of protein, MDA, and TOS remained unaltered.

Conclusions
Osteoarthritis is associated with oxidative stress and altered activities of the antioxidant enzymes in synovial membrane. However, lipid peroxidation seems not to be important for the etiology and development of osteoarthritis.

keywords:

synovial membrane, osteoarthritis, oxidative stress, antioxidant enzymes, malondialdehyde
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