Current issue
Archive
About the journal
Editorial board
Abstracting and indexing
Subscription
Contact
Instructions for authors
Publication charge
Ethical standards and procedures
Editorial System
Submit your Manuscript
|
4/2023
vol. 104 abstract:
RESEARCH PAPERS
Analysis of Prussian carp (Carassius gibelio B.) oocytes under the influence of Roundup® herbicide
KLAUDIA KWIATKOWSKA
1
BioTechnologia vol. 104(4) ∙ pp. 351–358 ∙ 2023
Online publish date: 2023/12/21
View
full text
Get citation
ENW EndNote
BIB JabRef, Mendeley
RIS Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
The aim of this study was to investigate the effect of Roundup® herbicide on the maturation of Prussian carp oocytes under laboratory conditions. The Prussian carp is currently one of the most common fish species in Polish freshwater ichthyofauna. For the investigation, oocytes from five sexually mature female Prussian carp were used, segmented into three groups, and incubated for 24 h in Cortland’s saline, treated with varied concentrations of the herbicide Roundup® (0 ng – control, R1 – 10 ng/ml, and R2 – 100 ng/ml). Subsequent to this period, assays were performed using the prepared plates to determine the level of 17α,20β-dihydroxyprogesterone (17α,20β-P) utilizing the standard ELISA technique. In determining the 17α,20β-P via ELISA, the medium was extracted from each tested oocyte group. Oocyte maturity was assessed through preservation in serra fluid, and, to categorize the maturity stage of the oocytes utilizing a four-point scale – contingent upon the nucleus’s position – the formerly preserved oocytes were dehydrated and subsequently analyzed. A contrast was noted in the percentage of oocytes at varied stages between the control group and the experimental groups. Specifically, a higher concentration of Roundup® (100 ng/ml) accelerated to expedite the initial migration of the nucleus in oocytes. In conclusion, the obtained results show the adverse effect of Roundup® on hormonal regulation and maturation in Prussian carp oocytes.
keywords:
glyphosate, ecotoxicology, herbicides, environmental protection |