Arsenic trioxide downregulates cancer procoagulant activity in MCF-7 and WM-115 cell lines <i>in vitro</i>

Ewelina A. Hoffman; Katarzyna Gizelska; Marek Mirowski; Wojciech Mielicki

doi:10.5114/wo.2014.41390

eISSN: 1897-4309
ISSN: 1428-2526

Contemporary Oncology/Współczesna Onkologia

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2/2015
vol. 19

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abstract:

Original paper

Arsenic trioxide downregulates cancer procoagulant activity in MCF-7 and WM-115 cell lines in vitro

Ewelina A. Hoffman

,

Katarzyna Gizelska

,

Marek Mirowski

,

Wojciech Mielicki

Contemp Oncol (Pozn) 2015; 19 (2): 108–112

DOI: https://doi.org/10.5114/wo.2014.41390

Online publish date: 2015/05/13

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The aim of the study: To analyze human breast cancer cell line MCF-7 and human malignant melanoma cell line WM-115 in order to characterize the cellular expression of CP and to evaluate whether ATO may affect this activity, as well as the viability of the cells.

Material and methods: The inhibitory effect of arsenic trioxide on the proliferation of MCF-7 and WM-115 cells were measured with MTT test. The activity of cancer procoagulant after ATO exposure was determined by a specific three-stage chromogenic assay.

Results: ATO decreased the CP activity in a dose- and time-dependent manner in MCF-7 cells with no effect on cell proliferation at the same time. However, it affected the CP activity of WM-115 cells in a different way. Reduction in CP activity was followed by an increase after 48 h incubation. The cells viability results showed dose- and time-correlated response within high arsenic concentrations.

Conclusions: Arsenic trioxide downregulates the CP expression in human breast cancer and melanoma cells.

keywords:

Arsenic trioxide downregulates cancer procoagulant activity in MCF-7 and WM-115 cell lines in vitro

Ewelina A. Hoffman , Katarzyna Gizelska , Marek Mirowski , Wojciech Mielicki

arsenic trioxide, cancer procoagulant, breast cancer, melanoma

Ewelina A. Hoffman

,

Katarzyna Gizelska

,

Marek Mirowski

,

Wojciech Mielicki