C-erbB2 (Her-2/neu) gene amplification in women with breast cancer – initial studies

Andrzej Kulig; Beata Smolarz; Elżbieta Kozłowska; Hanna Romanowicz-Makowska; Marek Zadrożny

eISSN: 2299-0038
ISSN: 1643-8876

Menopause Review/Przegląd Menopauzalny

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1/2002
vol. 1

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abstract:

C-erbB2 (Her-2/neu) gene amplification in women with breast cancer – initial studies

Andrzej Kulig

,

Beata Smolarz

,

Elżbieta Kozłowska

,

Hanna Romanowicz-Makowska

,

Marek Zadrożny

(Prz Menopauz 2002; 1: 11–14)

Online publish date: 2004/03/03

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Background. The c-erbB2 (Her2/neu) oncogene is located on the long arm of chromosome 7, encoding for a 185 kD transmembrane glycoprotein p185 with tyrosine kinase receptor function. Several studies have shown that overexpression of c-erbB2 (Her-2/neu) gene has been associated with breast cancer appearance. The aim of the present study was to investigate the relationship between amplification of c-erbB2 gene and the appearance of breast cancer in menopausal women.
Materials and methods. Paraffin embedded tumour tissue were obtained from patients with breast cancer (n=20). Blood samples from age matched healthy women (n=20) served as control. The amplification of c-erbB2 gene was determined by PCR reaction using the allele specific primers.
Results. The amplification of c-erbB2 gene was significantly higher in patients compared as control (p<0.05).
Conclusion. The results suggest that amplification of c-erbB2 gene may be linked with appearance of breast cancer in menopausal women.

keywords:

C-erbB2 (Her-2/neu) gene amplification in women with breast cancer – initial studies

Andrzej Kulig , Beata Smolarz , Elżbieta Kozłowska , Hanna Romanowicz-Makowska , Marek Zadrożny

c-erbB2 (Her-2/neu) gene, amplification, breast carcinoma, menopause, PCR

Andrzej Kulig

,

Beata Smolarz

,

Elżbieta Kozłowska

,

Hanna Romanowicz-Makowska

,

Marek Zadrożny