eISSN: 2354-0265
ISSN: 2353-6942
Health Problems of Civilization Physical activity: diseases and issues recognized by the WHO
Current issue Archive Online first About the journal Editorial board Reviewers Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
2/2020
vol. 14
 
Share:
Share:
abstract:
Review paper

CURRENT METHODS USED TO IDENTIFY AND GENOTYPE SPIROCHAETES BORRELIELLA BURGDORFERI

Patrycja Teodorowicz
1
,
Marcin Weiner
2

  1. Medical Laboratory, Innovation Research Center, Pope John Paul II State School of Higher Education in Biała Podlaska, Poland
  2. Faculty of Health Sciences, Pope John Paul II State School of Higher Education in Biała Podlaska, Poland
Health Prob Civil. 2020; 14(2): 71-82.
Online publish date: 2020/04/22
View full text Get citation
 
PlumX metrics:
Lyme disease, as one of tick-borne diseases, has been a current epidemiological problem in Poland and in the world for several years. The proportion of borreliosis infections caused by B. burgdorferi spirochaetes is increasing. Difficulties diagnosing this disease with conventional methods have led to growing molecular biology research aimed at developing improved diagnostic tools. Molecular biology methods include all techniques that allow the detection and analysis of nucleic acids. Among them there are methods based on PCR reaction, molecular typing methods (MLST), new generation sequencing (NGS). The current development of this field gives great research opportunities. With regard to B. burgdorferi spirochete, molecular biology is used to: confirm Lyme borreliosis, identify and type Borreliella spirochetes, detect them in tick vectors or intermediate hosts, as well as to identify co-infections between different Borreliella species and other tick-borne pathogens. They are meant to significantly improve diagnostic analyzes. This paper reviews the current methods used for the detection and identification of B. burgdorferi. Molecular mechanisms for the survival of spirochetes in the host, infection vectors and clinical picture of Lyme disease were also discussed.
keywords:

DNA, PCR, RNA, Borreliella burgdorferi, genospecies


Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.