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ISSN: 0860-7796
BioTechnologia
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4/2016
vol. 97
 
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abstract:
RESEARCH PAPERS

Callus induction and plant regeneration of Valeriana officinalis are affected by different leaf explants and various concentrations of plant growth regulators

Akram Zamini
1
,
Arash Mokhtari
2
,
Minoo Tansaz
3
,
Mohsen Zarei
2

  1. Department of Agricultural Biotechnology, Payam Noor University of Isfahan, Isfahan, Iran
  2. Agricultural Biotechnology Research Institute of Iran, Agricultural Research, Education and Extension Organization (AREEO), Tehran, Iran
  3. Department of Agricultural Biotechnology, Payam Noor University of Karaj, Karaj, Iran
BioTechnologia vol. 97(4) C pp. 261-269 C 2016
DOI: https://doi.org/10.5114/bta.2016.64543
Online publish date: 2017/01/13
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Valeriana officinalis, commonly known as valerian, is native to Europe and Asia and has economic importance as a medicinal plant. The effects of auxins (2,4-D and NAA) on callus induction were studied in vitro using basal piece of leaf + petiole (L1) and middle piece of leaf (L2) explants of V. officinalis. The best callus formation (95.83%) was reached on MS containing 2,4-D (1.5 mg/l!1) + KIN (1 mg/l!1) although there was no significant difference with 2,4-D (2 mg/l!1) + KIN (0.5 or 1 mg/l!1) on L2 explants. All calli successfully developed roots on callus induction media, a feature that can be used in the future for a large-scale production, saving time and cost. Among cytokinins (BAP, TDZ, and KIN) tested for callus differentiation, the highest regeneration (62.5%), shoot number (5.87) and shoot length (5.96 cm), occurred on a combination of BAP (0.5 mg/l!1) + IBA (0.5 mg/l!1). The results revealed that the effect of explant type on callogenesis was also significant. In contrast to KIN or TDZ, addition of IBA to BAP showed positive synergistic effect for plant regeneration and shoot number per callus.
keywords:

callogenesis; plant growth regulators; shoot regeneration; valerian


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