eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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3/2020
vol. 45
 
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abstract:
Clinical immunology

Changes of post-operative peripheral blood dendritic cells in patients undergoing laparoscopy

Kamil Torres
1
,
Łukasz Pietrzyk
1
,
Marta Denisow-Pietrzyk
1
,
Sebastian Radej
2
,
Andrzej Chrościcki
3
,
Anna Torres
2

  1. Department of Didactics and Medical Simulation, Chair of Human Anatomy, Medical University of Lublin, Poland
  2. Laboratory of Biostructure, Chair and Department of Human Anatomy, Medical University of Lublin, Poland
  3. Department of General and Oncological Surgery, District Specialist Hospital of Lublin, Poland
Cent Eur J Immunol 2020; 45 (3): 310-314
DOI: https://doi.org/10.5114/ceji.2020.101255
Online publish date: 2020/11/01
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Introduction
Surgical intervention affects local and systemic immune responses, especially in obese individuals. Many studies have attempted to evaluate immunological response to surgical trauma. Surgery changes the quantity and phenotype of circulating blood dendritic cells (DCs), including a decrease of total DCs post-operatively. The study aimed to evaluate the percentage and changes of myeloid, lymphoid DCs, and myeloid to lymphoid DCs ratio in obese and normal weight patients undergoing laparoscopy.

Material and methods
The study enrolled asymptomatic patients with gallstones, who underwent laparoscopic cholecystectomy. Blood samples were obtained before the surgery as well as 24 and 48 hours after the surgery. Cells were collected using a FACSCalibur flow cytometry, and phenotypes were analyzed with CellQuest software.

Results
No statistically significant differences were observed between obese and normal-weighted patients in all studied time periods, except for the myeloid to lymphoid DCs ratio assessed at 48-post-operative hour. The myeloid DCs percentage increased significantly in the post-operative period within both studied groups. The percentage of lymphoid DCs increased significantly in obese patients in all studied time periods.

Conclusions
Laparoscopy induces immunomodulation, such as changes of myeloid and lymphoid dendritic cells, especially in obese patients. We describe new findings, in which minimally invasive surgical trauma promotes the increase of percentage of circulating DCs in the early post-operative period.

keywords:

dendritic cells, obesity, laparoscopy, surgery, immune system
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