eISSN: 2084-9869
ISSN: 1233-9687
Polish Journal of Pathology
Current issue Archive Manuscripts accepted About the journal Supplements Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
3/2024
vol. 75
 
Share:
Share:
abstract:
Original paper

CircRNA TUBA1C promotes proliferation and glucose metabolism, and blocks apoptosis of osteosarcoma cells through sponging miR-143-3p

Yi-Bing Liu
1
,
Wen-Hao Dai
1
,
Jun-Jie Chang
1
,
Kang Wei
1

  1. Department of Orthopaedics, Changzhou TCM Hospital of Nanjing University of Chinese Medicine, Changzhou City, Jiangsu Province, China
Pol J Pathol 2024; 75 (3): 215-227
Online publish date: 2024/10/16
View full text Get citation
 
PlumX metrics:
Osteosarcoma (OS) is a malignant bone tumour that commonly occurs in paediatric and adolescent patients. Currently, effective therapy for OS remains elusive due to poor patient survival rates.

In this study, we observed significantly elevated expressions of circTUBA1C in OS tumours and cells.

Silencing circTUBA1C effectively suppressed proliferation and glucose metabolism, and promoted apoptosis of OS cells. Furthermore, we discovered that miR-143-3p played a reverse role to circTUBA1C in OS cells. Bioinformatics analysis, RNA pull-down assay, and luciferase assay demonstrated that circTUBA1C acted as a sponge for miR-143-3p, blocking its expression in OS cells. Finally, rescue experiments showed that inhibition of miR-143-3p in circTUBA1C-silenced OS cells significantly overrode the low-circTUBA1C-mediated miR-143-3p upregulation and OS cell progression in vitro and in vivo.

Our results demonstrate the critical roles and molecular targets of circTUBA1C in modulating OS progression, suggesting that circTUBA1C inhibition could serve as a new therapeutic strategy for treating OS.
keywords:

osteosarcoma, circular RNA, circTUBA1C, miR-143-3p, cell proliferation, glucose metabolism, cell apoptosis

Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.