Clinical immunology<br> The influence of IL-6 and TNF-α genes  promoter region polymorphisms (G174C – IL-6 and G308A – TNF-α) on the level of cytokines in obese and normal weight subjects

Katarzyna Popko; Elżbieta Górska; Anna Stokłosa; Robert Pływaczewski; Dorota Górecka; Marcin Popko; Tadeusz Szwed; Paweł Śliwiński; Maria Wąsik; Urszula Demkow

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ISSN: 1426-3912

Central European Journal of Immunology

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abstract:

Clinical immunology
The influence of IL-6 and TNF-α genes promoter region polymorphisms (G174C – IL-6 and G308A – TNF-α) on the level of cytokines in obese and normal weight subjects

Katarzyna Popko

,

Elżbieta Górska

,

Anna Stokłosa

,

Robert Pływaczewski

,

Dorota Górecka

,

Marcin Popko

,

Tadeusz Szwed

,

Paweł Śliwiński

,

Maria Wąsik

,

Urszula Demkow

Centr Eur J Immunol 2010; 35 (1): 31-36

Online publish date: 2010/04/06

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AMA

Popko K, Górska E, Stokłosa A, et al. Clinical immunology The influence of IL-6 and TNF-α genes  promoter region polymorphisms (G174C – IL-6 and G308A – TNF-α) on the level of cytokines in obese and normal weight subjects. Central European Journal of Immunology. 2010;35(1):31-36.

APA

Popko, K., Górska, E., Stokłosa, A., Pływaczewski, R., Górecka, D.,  & Popko, M. et al. (2010). Clinical immunology The influence of IL-6 and TNF-α genes  promoter region polymorphisms (G174C – IL-6 and G308A – TNF-α) on the level of cytokines in obese and normal weight subjects. Central European Journal of Immunology, 35(1), 31-36.

Chicago

Popko, Katarzyna, Elżbieta Górska, Anna Stokłosa, Robert Pływaczewski, Dorota Górecka, Marcin Popko,  and Tadeusz Szwed et al. 2010. "Clinical immunology The influence of IL-6 and TNF-α genes  promoter region polymorphisms (G174C – IL-6 and G308A – TNF-α) on the level of cytokines in obese and normal weight subjects". Central European Journal of Immunology 35 (1): 31-36.

Harvard

Popko, K., Górska, E., Stokłosa, A., Pływaczewski, R., Górecka, D., Popko, M., Szwed, T., Śliwiński, P., Wąsik, M.,  and Demkow, U. (2010). Clinical immunology The influence of IL-6 and TNF-α genes  promoter region polymorphisms (G174C – IL-6 and G308A – TNF-α) on the level of cytokines in obese and normal weight subjects. Central European Journal of Immunology, 35(1), pp.31-36.

MLA

Popko, Katarzyna et al. "Clinical immunology The influence of IL-6 and TNF-α genes  promoter region polymorphisms (G174C – IL-6 and G308A – TNF-α) on the level of cytokines in obese and normal weight subjects." Central European Journal of Immunology, vol. 35, no. 1, 2010, pp. 31-36.

Vancouver

Popko K, Górska E, Stokłosa A, Pływaczewski R, Górecka D, Popko M et al. Clinical immunology The influence of IL-6 and TNF-α genes  promoter region polymorphisms (G174C – IL-6 and G308A – TNF-α) on the level of cytokines in obese and normal weight subjects. Central European Journal of Immunology. 2010;35(1):31-36.

Proinflammatory cytokines level, including IL-6 and TNF-α, is changing significantly during hyperplasia and hypertrophy of adipocytes. The increase in IL-6 and TNF-α concentration is a direct result of increased expression of their genes. Gene polymorphisms, especially those localized in transcription regulating regions such as promoters, are among factors influencing gene expression profile. The aim of the study is to assess the influence of polymorphic changes in promoter regions: G174C in IL-6 gene and G308A in TNF-α gene on the level of the cytokines in obese and normal weight subjects. The study included 102 obese subjects and 77 normal weight subjects. Genotyping was performed using PCR-RFLP technique, while cytokine level was assessed using ELISA assay. The obtained results showed the connection between a certain genotype and IL-6 and TNF-α genes expression level. In non obese subjects with C/C genotype IL-6 level was lower than in subjects with G/C genotypes. In contrary in TNF-α gene the difference was found in the group of obese. The A allel carriers were characterized by higher TNF concentration. However, many additional modifying factors, which often conceal the influence of polymorphisms on gene expression, should be considered.

keywords:

Clinical immunology The influence of IL-6 and TNF-α genes promoter region polymorphisms (G174C – IL-6 and G308A – TNF-α) on the level of cytokines in obese and normal weight subjects

Katarzyna Popko , Elżbieta Górska , Anna Stokłosa , Robert Pływaczewski , Dorota Górecka , Marcin Popko , Tadeusz Szwed , Paweł Śliwiński , Maria Wąsik , Urszula Demkow

obesity, interleukin-1, interleukin-6, tumor necrosis factor α, gene polymorphism

Clinical immunology
The influence of IL-6 and TNF-α genes promoter region polymorphisms (G174C – IL-6 and G308A – TNF-α) on the level of cytokines in obese and normal weight subjects

Katarzyna Popko

,

Elżbieta Górska

,

Anna Stokłosa

,

Robert Pływaczewski

,

Dorota Górecka

,

Marcin Popko

,

Tadeusz Szwed

,

Paweł Śliwiński

,

Maria Wąsik

,

Urszula Demkow