Clinical immunology<br>The metabolic activity of neutrophils measured with a chemiluminescence assay in young patients with type 1 diabetes depending 
on the disease duration

Magdalena Kaczmarek; Zdzisława Iwanicka; Adam Jankowski; Aleksandra Lewandowicz-Uszyńska

doi:10.5114/ceji.2013.35218

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures

Editorial System

Submit your Manuscript

2/2013
vol. 38

Send email

Copy url:

abstract:

Clinical immunology
The metabolic activity of neutrophils measured with a chemiluminescence assay in young patients with type 1 diabetes depending on the disease duration

Magdalena Kaczmarek

,

Zdzisława Iwanicka

,

Adam Jankowski

,

Aleksandra Lewandowicz-Uszyńska

(Centr Eur J Immunol 2013; 38 (2): 237-242)

DOI: https://doi.org/10.5114/ceji.2013.35218

Online publish date: 2013/07/08

View full text Get citation

EndNote

Papers, Reference Manager, RefWorks, Zotero

AMA

Kaczmarek M, Iwanicka Z, Jankowski A, Lewandowicz-Uszyńska A. Clinical immunologyThe metabolic activity of neutrophils measured with a chemiluminescence assay in young patients with type 1 diabetes depending 
on the disease duration. Central European Journal of Immunology. 2013;38(2):237-242. doi:10.5114/ceji.2013.35218.

APA

Kaczmarek, M., Iwanicka, Z., Jankowski, A.,  & Lewandowicz-Uszyńska, A. (2013). Clinical immunologyThe metabolic activity of neutrophils measured with a chemiluminescence assay in young patients with type 1 diabetes depending 
on the disease duration. Central European Journal of Immunology, 38(2), 237-242. https://doi.org/10.5114/ceji.2013.35218

Chicago

Kaczmarek, Magdalena, Zdzisława Iwanicka, Adam Jankowski,  and Aleksandra Lewandowicz-Uszyńska. 2013. "Clinical immunologyThe metabolic activity of neutrophils measured with a chemiluminescence assay in young patients with type 1 diabetes depending 
on the disease duration". Central European Journal of Immunology 38 (2): 237-242. doi:10.5114/ceji.2013.35218.

Harvard

Kaczmarek, M., Iwanicka, Z., Jankowski, A.,  and Lewandowicz-Uszyńska, A. (2013). Clinical immunologyThe metabolic activity of neutrophils measured with a chemiluminescence assay in young patients with type 1 diabetes depending 
on the disease duration. Central European Journal of Immunology, 38(2), pp.237-242. https://doi.org/10.5114/ceji.2013.35218

MLA

Kaczmarek, Magdalena et al. "Clinical immunologyThe metabolic activity of neutrophils measured with a chemiluminescence assay in young patients with type 1 diabetes depending 
on the disease duration." Central European Journal of Immunology, vol. 38, no. 2, 2013, pp. 237-242. doi:10.5114/ceji.2013.35218.

Vancouver

Kaczmarek M, Iwanicka Z, Jankowski A, Lewandowicz-Uszyńska A. Clinical immunologyThe metabolic activity of neutrophils measured with a chemiluminescence assay in young patients with type 1 diabetes depending 
on the disease duration. Central European Journal of Immunology. 2013;38(2):237-242. doi:10.5114/ceji.2013.35218.

PlumX metrics:

The study deals with the estimation of the metabolic activity of neutrophils measured with a chemiluminescence assay in children with type I diabetes depending on the disease duration, compared to the control group. The study involved 87 children with type I diabetes aged 4 to 21 years (52 girls, 35 boys). Patients were divided into four subgroups depending on the disease duration: subgroup I – the disease duration up to 12 months (35 children), subgroup II – the disease duration up to 5 years (28 children), subgroup III – the disease duration up to 10 years (12 children), and subgroup IV – the disease duration over 10 years (12 children). The control group was composed of 23 persons – healthy volunteers aged 20 to 25 years (mean age 23 years). The metabolic activity of neutrophils was determined in a luminol-dependent chemiluminescence assay. The spontaneous and stimulated chemiluminescence of

neutrophils (fMLP, opsonised Zymosan, PMA) was tested on a 96-well microplate (LB96P-WMP).

The chemiluminescence assay was conducted using a luminometer (MicroLumat LB 96P of E&G BARTHOLD). The results have been written in RLU units (Relative Light Units/s) as the maximum value of chemiluminescence (CLmax). The test showed statistically significantly lower values of spontaneous and stimulated neutrophil chemiluminescence in all subgroups, compared to the control group. The lowest levels of neutrophil CL were in subgroup III (neutrophils stimulated with fMLP and PMA) and also in children with newly detected diabetes (Zymosan-stimulated neutrophils).

keywords:

Clinical immunologyThe metabolic activity of neutrophils measured with a chemiluminescence assay in young patients with type 1 diabetes depending on the disease duration

Magdalena Kaczmarek , Zdzisława Iwanicka , Adam Jankowski , Aleksandra Lewandowicz-Uszyńska

diabetes, children, neutrophil chemiluminescence, disease duration

Clinical immunology
The metabolic activity of neutrophils measured with a chemiluminescence assay in young patients with type 1 diabetes depending on the disease duration

Magdalena Kaczmarek

,

Zdzisława Iwanicka

,

Adam Jankowski

,

Aleksandra Lewandowicz-Uszyńska