eISSN: 2449-8238
ISSN: 2392-1099
Clinical and Experimental Hepatology
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4/2018
vol. 4
 
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abstract:
Original paper

Diagnosis of spontaneous bacterial peritonitis in children using leukocyte esterase reagent strips and granulocyte elastase immunoassay

Alif Abd El-Hakim Allam
,
Sara Mohammed Eltaras
,
Mohsen Hassan Hussin
,
El-Sayed Ibrahem Salama
,
Olfat Mohamed Hendy
,
Maha Moawad Allam
,
Haidy Mohammed Zakaria

Clin Exp HEPATOL 2018; 4, 4: 247–252
DOI: https://doi.org/10.5114/ceh.2018.80126
Online publish date: 2018/12/03
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Aim of the study
We aimed to assess the utility and rapidity of granulocyte elastase (GE) latex immunoassay and leukocyte esterase (LE) reagent strips for the diagnosis of spontaneous bacterial peritonitis (SBP) in hepatic children with ascites.

Material and methods
This study included 80 ascitic fluid (AF) samples from 45 patients with chronic liver diseases. They were divided into 2 groups (SBP and non-SBP groups). White blood cells > 500 cell/mm3 or polymorphonuclear leukocytes > 250 cell/mm3 were considered as a positive result for the diagnosis of SBP. The AF obtained at bedside was immediately tested with an LE enzyme reagent strip, and another sample was aliquoted for measurement of serum GE.

Results
On doing LE dipstick strips, all the non-SBP group gave no coloration with LE strips while 62% of the SBP group gave coloration. LE strips had accuracy of 86.25% in differentiating SBP and non-SBP at a cut-off value of 1 (color grade 1). The diagnostic performance of GE in differentiating SBP and non-SBP was assessed and showed accuracy of 70% for a cutoff value of 123.5 ng/ml.

Conclusions
Application of LE reagent strips is a bedside, rapid, easy-to-use, and inexpensive method for diagnosis of SBP. It has an accuracy of 86.25% in differentiating SBP and non-SBP, which is higher than more complicated and delayed methods such as GE latex immunoassay, which has an accuracy of 70%.

keywords:

spontaneous bacterial peritonitis, granulocyte elastase, leukocyte esterase
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