Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A

Beniamin Grabarek; Piotr Schweizer; Iwona Adwent; Dominika Wcisło-Dziadecka; Jakub Krzaczyński; Celina Kruszniewska-Rajs; Joanna Gola

doi:10.5114/ada.2019.91506

eISSN: 2299-0046
ISSN: 1642-395X

Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii

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Original paper

Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A

Beniamin Grabarek

^{1, 2, 3}

,

Piotr Schweizer

¹

,

Iwona Adwent

¹

,

Dominika Wcisło-Dziadecka

⁴

,

Jakub Krzaczyński

³

,

Celina Kruszniewska-Rajs

³

,

Joanna Gola

³

Katowice School of Technology, The University of Science and Art, Katowice, Poland
Centre of Oncology, M. Sklodowska-Curie Memorial Institute, Cracow Branch, Poland
Department of Molecular Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Poland
Department of Cosmetology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia in Katowice, Poland

Adv Dermatol Allergol 2021; XXXVIII (2): 249-255

DOI: https://doi.org/10.5114/ada.2019.91506

Online publish date: 2020/01/09

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AMA

Grabarek B, Schweizer P, Adwent I, et al. Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2021;38(2):249-255. doi:10.5114/ada.2019.91506.

APA

Grabarek, B., Schweizer, P., Adwent, I., Wcisło-Dziadecka, D., Krzaczyński, J.,  & Kruszniewska-Rajs, C. et al. (2021). Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 38(2), 249-255. https://doi.org/10.5114/ada.2019.91506

Chicago

Grabarek, Beniamin, Piotr Schweizer, Iwona Adwent, Dominika Wcisło-Dziadecka, Jakub Krzaczyński, Celina Kruszniewska-Rajs,  and Joanna Gola. 2021. "Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A". Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii 38 (2): 249-255. doi:10.5114/ada.2019.91506.

Harvard

Grabarek, B., Schweizer, P., Adwent, I., Wcisło-Dziadecka, D., Krzaczyński, J., Kruszniewska-Rajs, C.,  and Gola, J. (2021). Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 38(2), pp.249-255. https://doi.org/10.5114/ada.2019.91506

MLA

Grabarek, Beniamin et al. "Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A." Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, vol. 38, no. 2, 2021, pp. 249-255. doi:10.5114/ada.2019.91506.

Vancouver

Grabarek B, Schweizer P, Adwent I, Wcisło-Dziadecka D, Krzaczyński J, Kruszniewska-Rajs C et al. Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2021;38(2):249-255. doi:10.5114/ada.2019.91506.

Introduction
Adalimumab and cyclosporine A are drugs used in moderate to severe forms of psoriasis. Despite the molecular orientation of the drugs, there is a loss of adequate cell sensitivity to the anti-cytokine therapy.

Aim
To determine the changes in the gene expression profile associated with drug resistance in the culture of normal human dermal fibroblasts (NHDF) exposed to adalimumab or cyclosporine A compared to the controls.

Material and methods
NHDF was exposed to adalimumab/cyclosporine A for 2, 8, 24 h compared to the control culture. Molecular analysis was performed using mRNA and miRNA microarray techniques. The obtained results were analysed using PL – Grid infrastructure (p < 0.05).

Results
Of the 22277 ID mRNA, 47 are associated with drug resistance, of which the change in expression of 17 mRNA ID is statistically significant (p < 0.05). The greatest change in transcriptional activity (FC ≥ 1.3) was observed for GLO1, SLC10A3, TUFT1, STATH, ABCB1, AGTR1. Expression of these genes can be regulated by miR-199a-5p, miR-1231, miR-34a, miR-3188, and miR-106a (except AGTR1).

Conclusions
The analysis of changes in the expression of mRNA and miRNA related to drug resistance gives the possibility of monitoring the effectiveness of anti-cytokine therapy.

keywords:

Differences in expression of genes related to drug resistance and miRNAs regulating their expression in skin fibroblasts exposed to adalimumab and cyclosporine A

Beniamin Grabarek 1, 2, 3 , Piotr Schweizer 1 , Iwona Adwent 1 , Dominika Wcisło-Dziadecka 4 , Jakub Krzaczyński 3 , Celina Kruszniewska-Rajs 3 , Joanna Gola 3

psoriasis, drug resistance, personalized therapy, miRNA

Beniamin Grabarek

^{1, 2, 3}

,

Piotr Schweizer

¹

,

Iwona Adwent

¹

,

Dominika Wcisło-Dziadecka

⁴

,

Jakub Krzaczyński

³

,

Celina Kruszniewska-Rajs

³

,

Joanna Gola

³