Effect of acute isooxic hypercapnia
on oxidative activity of systemic neutrophils
in endotoxemic rabbits

Hanna Billert; Ewa Bednarek; Krzysztof Kusza; Mirosław Ponichter; Maciej Kurpisz

doi:10.5114/ceji.2021.105245

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures

Editorial System

Submit your Manuscript

1/2021
vol. 46

Send email

Copy url:

abstract:

Experimental immunology

Effect of acute isooxic hypercapnia on oxidative activity of systemic neutrophils in endotoxemic rabbits

Hanna Billert

¹

,

Ewa Bednarek

¹

,

Krzysztof Kusza

²

,

Mirosław Ponichter

¹

,

Maciej Kurpisz

³

Department of Experimental Anaesthesiology, Chair of Anaesthesiology and Intensive Therapy, Poznan University of Medical Sciences, Poznan, Poland
Clinics of Anaesthesiology and Intensive Therapy, Chair of Anaesthesiology and Intensive Therapy, Poznan University of Medical Sciences, Poznan, Poland
Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

Cent Eur J Immunol 2021; 46 (1): 47-53

DOI: https://doi.org/10.5114/ceji.2021.105245

Online publish date: 2021/04/18

View full text Get citation

PlumX metrics:

Introduction
Whether carbon dioxide (CO2) affects systemic oxidative phenomena under conditions of endotoxemia is not sufficiently clarified. The study aimed to assess the impact of moderate acute hypercapnia on the respiratory burst of circulating neutrophils in mechanically ventilated endotoxemic rabbits.

Material and methods
Twenty-four endotoxemic rabbits were mechanically ventilated with standard or CO2‑enriched gas mixture in order to obtain isooxic hypercapnia. At a baseline point and following 180 min of hypercapnic ventilation, luminol-dependent chemiluminescence (CL) of circulating neutrophils and serum 2-thiobarbituric acid reactive substance (TBARS) concentrations were measured. Throughout the study, leukocyte and neutrophil counts, pH status, circulatory parameters and body temperature were also assessed.

Results
Following 180 min of hypercapnic ventilation, opsonized zymosan (OZ)-stimulated neutrophils showed lower CL vs. the control group (p = 0.004). Other parameters studied were not affected.

Conclusions
Short-term isooxic hypercapnia in endotoxemic rabbits preserves circulating neutrophil count pattern and reactive oxygen species (ROS) generation, but it may reduce phagocytosis.

keywords:

Effect of acute isooxic hypercapnia on oxidative activity of systemic neutrophils in endotoxemic rabbits

Hanna Billert 1 , Ewa Bednarek 1 , Krzysztof Kusza 2 , Mirosław Ponichter 1 , Maciej Kurpisz 3

endotoxemia, hypercapnia, respiratory burst, circulating neutrophils

Hanna Billert

¹

,

Ewa Bednarek

¹

,

Krzysztof Kusza

²

,

Mirosław Ponichter

¹

,

Maciej Kurpisz

³