eISSN: 2299-551X
ISSN: 0011-4553
Journal of Stomatology
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1/2023
vol. 76
 
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abstract:
Original paper

Effect of duck eggshell nano-calcium carbonate and phosphopeptide-amorphous calcium phosphate casein on re-mineralization of deciduous enamel surface

Muhammad Chair Effendi
1
,
Btary Bella Victory
2
,
Ellyza Herda
3
,
Mutiara Fauzia Nurmawlidina
1
,
Ahmad Taufiq
4

  1. Department of Pediatric Dentistry, Universitas Brawijaya, Malang, Indonesia
  2. Undergraduate Program in Dentistry, Universitas Brawijaya, Malang, Indonesia
  3. Department of Dental Material, Universitas Indonesia, Jakarta, Indonesia
  4. Department of Physics, Universitas Negeri Malang, Malang, Indonesia
J Stoma 2023; 76, 1: 1-9
Online publish date: 2022/12/22
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Introduction
Dental caries has been the most prevalent disease in humans, particularly in children. OBJECTIVES: This study aimed to determine the difference between duck eggshell’s nano-calcium carbonate (NCDe) and casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) in increasing enamel re-mineralization

Material and methods
5% and 10% NCDe were obtained from 50 g of thick NCDe; then, 2.5 g were taken with 2.5 g of carboxymethyl cellulose (CMC) and 45 ml of distilled water to obtain 5% NCDe gel. Twenty-four primary maxillary first incisor teeth were divided into four groups, i.e., (1) negative control, (2) posi­tive control (10% CPP-ACP), (3) 5% NCDe, and (4) 10% NCDe. Each tooth sample was measured for the enamel surface’s micro-hardness using Vickers hardness test. Meanwhile, the enamel surface morphology was captured by means of electron microscopy scanning with magnification of 5,000×.

Results
The average micro-hardness of the enamel in the 10% NCDe and 10% CPP-ACP groups were 343.66 HV and 305.28 HV, respectively. In addition, the 10% NCDe group showed the lowest enamel porosity with strongest positive correlation between treatments’ duration, with an increase in enamel re-mineralization. In terms of statistical data, the correlation and linearity parameters for the 10% NCDe group were .800 and 64.1%, respectively.

Conclusions
The 10% NCDe group tended to be more effective than 5% NCDe and 10 CPP-APP groups in increasing re-mineralization of tooth enamel. Therefore, it was confirmed that NCDe 10% group has met the criteria as an excellent agent to re-mineralize primary teeth enamel.

keywords:

hardness, calcium carbonate, tooth re-mineralization, dental enamel

 
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