Effect of eccentric action velocity on expression of genes related to
myostatin signaling pathway in human skeletal muscle

Hamilton Roschel; Carlos Ugrinowistch; Audrei Reis Santos; Wesley Pereira Barbosa; Elen Haruka Miyabara; Valmor Tricoli; Marcelo Saldanha Aoki

doi:10.5114/biolsport.2018.71600

eISSN: 2083-1862
ISSN: 0860-021X

Biology of Sport

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2/2018
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abstract:

Original paper

Effect of eccentric action velocity on expression of genes related to myostatin signaling pathway in human skeletal muscle

Hamilton Roschel

,

Carlos Ugrinowistch

,

Audrei Reis Santos

,

Wesley Pereira Barbosa

,

Elen Haruka Miyabara

,

Valmor Tricoli

,

Marcelo Saldanha Aoki

Biol Sport. 2018;35(2):111–119

DOI: https://doi.org/10.5114/biolsport.2018.71600

Online publish date: 2018/02/06

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The aim of this study was to investigate the effects of an acute bout of eccentric actions, performed at fast velocity (210º^.s-1) and at slow velocity (20º^.s-1), on the gene expression of regulatory components of the myostatin (MSTN) signalling pathway. Participants performed an acute bout of eccentric actions at either a slow or a fast velocity. Muscle biopsy samples were taken before, immediately after, and 2 h after the exercise bout. The gene expression of the components of the MSTN pathway was assessed by real-time PCR. No change was observed in MSTN, ACTRIIB, GASP-1 or FOXO-3a gene expression after either slow or fast eccentric actions (p > 0.05). However, the MSTN inhibitors follistatin (FST), FST-like-3 (FSTL3) and SMAD-7 were significantly increased 2 h after both eccentric actions (p < 0.05). No significant difference between bouts was found before, immediately after, or 2 h after the eccentric actions (slow and fast velocities, p > 0.05). The current findings indicate that a bout of eccentric actions activates the expression of MSTN inhibitors. However, no difference was observed in MSTN inhibitors’ gene expression when comparing slow and fast eccentric actions. It is possible that the greater time under tension induced by slow eccentric (SE) actions might compensate the effect of the greater velocity of fast eccentric (FE) actions. Additional studies are required to address the effect of eccentric action (EA) velocities on the pathways related to muscle hypertrophy.

keywords:

Effect of eccentric action velocity on expression of genes related to myostatin signaling pathway in human skeletal muscle

Hamilton Roschel , Carlos Ugrinowistch , Audrei Reis Santos , Wesley Pereira Barbosa , Elen Haruka Miyabara , Valmor Tricoli , Marcelo Saldanha Aoki

Slow eccentric, Fast eccentric, Gene expression, GDF-8, Signalling

Hamilton Roschel

,

Carlos Ugrinowistch

,

Audrei Reis Santos

,

Wesley Pereira Barbosa

,

Elen Haruka Miyabara

,

Valmor Tricoli

,

Marcelo Saldanha Aoki