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Polish Journal of Pathology
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2/2023
vol. 74
 
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abstract:
Original paper

Effect of lentinan on proliferation and apoptosis of human astrocytoma U251 cells

Min Yuan
1
,
Chengyu Li
2
,
Xiang Xiao
3
,
Dengfeng Wan
3
,
Bin Xi
3
,
Xingxing Jiang
3
,
Jianzhong Zhang
3

  1. Department of Neurology, Jiangxi Provincial People’s Hospital Affiliated to Nanchang University, PR China
  2. Department of Neurosurgery, Jiangxi Qianshan County People's Hospital, PR China
  3. Department of Neurosurgery, Jiangxi Provincial People’s Hospital Affiliated to Nanchang University, PR China
POL J PATHOL 2023; 74 (2): 136-140
DOI: https://doi.org/10.5114/pjp.2022.119264
Online publish date: 2022/09/09
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Aim of the study
Aim of the study is To investigate the effect of lentinan on proliferation and apoptosis of human astrocytoma U251 cells. Lentinan was dissolved in DMEM complete medium to form different concentrations (0, 25, 50, 100, 200, 400, 500, 600 µg/ml). CCK8 was used to detect the effect of lentinan with different concentrations on proliferation of human astrocytoma U251 cells, and the expression of Ki-67 was detected by immunofluorescence. In addition, the effect of different concentrations of lentinan on apoptosis of human astrocytoma U251 cells was detected by flow cytometry. Compared with the blank control group, 50 and 100 µg/ml lentinan significantly promoted proliferation of human astrocytoma U251 cells. When the concentration is more than 100 µg/ml, the cell activity gradually decreases, and the cell activity is the lowest when the concentration is 600 µg/ml. In addition, the low concentration lentinan (25, 50, and 100 µg/ml) had no significant effect on apoptosis of human astrocytoma U251 cells. However, lentinan above 200 µg/ml significantly promoted apoptosis of human astrocytoma U251 cells and had a concentration gradient effect, and the highest apoptosis rate was at 600 µg/ml.

Conclusions
Lentinan can effectively inhibit proliferation and promote apoptosis of human astrocytoma U251 cells.

keywords:

lentinan, astrocytoma, U251 cells, cell proliferation, apoptosis
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