eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
1/2020
vol. 101
 
Share:
Share:
abstract:
RESEARCH PAPERS

Effects of thidiazuron on in vitro shoot regeneration of Morus alba

Mohammad Saleh Rezaei-Zafarghandi
1
,
Mostafa Rahmati-Joneidabad
2

  1. Department of Agronomy and Plant Breeding Science, College of Aburaihan, University of Tehran, Tehran-Pakdasht, Iran
  2. Department of Horticultural Science, Faculty of Agriculture, Agricultural Sciences and Natural Resources, University of Khuzestan, Mollasani, Iran
BioTechnologia vol. 101 (1) C pp. 55–61 C 2020
Online publish date: 2020/03/27
View full text Get citation
 
PlumX metrics:
Mulberry (Morus alba L.) is an ornamental, medicinal, and fruit plant that belongs to the Moraceae family. One of the most important techniques used in plant biotechnology is tissue culture, which enables mass production of pathogen-free plants. Cotyledon has a high potential for shoot regeneration; however, to the best of our knowledge, there are no reports on plant regeneration from cotyledon explants of mulberry. Therefore, this study aimed to evaluate the potential of seedling-derived cotyledon segments to obtain shoot multiplication of mulberry. Various concentrations (0, 0.25, 0.5, 1, 2, 3, and 4 mg/l) of thidiazuron (TDZ) in combination with indole butyric acid (IBA) were used in a completely randomized design in three replications. The results showed that the highest percentage of regeneration frequency (96.67%) and the maximum number of shoots (4.43) were obtained on Murashige and Skoog (MS) medium supplemented with 0.25 mg/l TDZ and 0.025 mg/l IBA. In the rooting experiment, the maximum rooting percentage (83.33%) and the maximum number of roots per shoot (4.36) were obtained on MS medium containing 2 mg/l IBA. In vitro-raised plantlets were placed in pots and kept in room temperature for 30 days, and the plantlets showed more than 90% survival rate. On the basis of our results, the protocol described in this study has a high potential to be used in the micropropagation of this valuable plant.
keywords:

mulberry; cotyledon; plant growth regulator; organogenesis; in vitro-grown seedlings



Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here