Effects of vitamin B12 on isolated tubal torsion with ischaemia/reperfusion injury: evaluation with histological and biochemical parameters

Aim
The aim of this study was to determine the histopathological and biochemical effects of vitamin B₁₂ on I/R injury in the rat isolated tubal torsion/detorsion model.

Material and methods
Twenty-eight rats were divided into a control group (group 1), torsion group (group 2), torsion + detorsion group (group 3), and torsion + detorsion + vitamin B₁₂ group (group 4). After the experimental procedure, histopathological evaluation was done. Superoxide dismutase (SOD) and glutathione peroxidase (GP-Px) enzyme activities were measured.

Results
As a result of histopathological evaluations, tuba uterina tissues were normal in group 1 and group 4. In group 2, epithelial degeneration and abnormal epithelial proliferation, cilia loss, and vascular congestion foci were observed. Histological examination of group 3 showed increased epithelial degeneration and abnormal epithelial proliferation and vascular congestion compared to group 2. There were findings of ischaemia and reperfusion injury in group 2 and group 3. In biochemical examinations, SOD and GSH-Px enzyme levels were significantly increased in group 1 and group 4 compared to group 3 (p < 0.05).

Conclusions
It has been experimentally demonstrated that vitamin B₁₂ reduces I/R damage in an isolated tubal torsion rat model. This is the first study on the effectiveness of vitamin B₁₂ on I/R damage in ITT.