eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
Newsletter
Enter your email:
 
Search
Editorial Policies
Sarajevo Declaration on Integrity and Visibility of Scholarly Publications

Without publication fees

without publication fees

Open access

without publication fees

Journal integrated with

integrated with publons
1/2016
vol. 97
 
Share:
Send email
Share:
abstract:
RESEARCH PAPERS

Enzyme-mediated protoplast formation of Cuninghamella echinulata – preliminary studies

Małgorzata Brzezińska-Rodak
1
,
Magdalena Klimek-Ochab
1
,
Ewa Żymańczyk-Duda
1

  1. Wrocław University of Technology, Faculty of Chemistry, Wrocław, Poland
BioTechnologia vol. 97(1) C pp. 27-32 C 2016
DOI: https://doi.org/10.5114/bta.2016.58541
Online publish date: 2016/04/20
View full text Get citation
 
PlumX metrics:
In this report, we have presented the analysis of the enzymatic methods of protoplasts formation of Cunninghamella echinulata. Hydrolytic enzymes of different origin (i.e. prokaryotic and eukaryotic) such as chitinase (EC.3.2.1.14), lyticase (mix of lytic enzymes), and proteinase K (EC 3.4.21.64) were used for fungal cell wall disruption. An effective protoplast formation was achieved with the following enzyme solutions: chitinase (0.33 U A ml!1), proteinase K (1.8-3.6 U A ml!1), and lyticase (160 UA ml!1). Fluorescent microscopy was used to evaluate the ability of the tested enzymes to effectively produce protoplasts.
keywords:

fungal protoplasts, Cuninghamella echinulata, protoplast formation


Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here
Hosted by Termedia sp. z o.o.