Estimation of salivary matrix metalloproteinase-9 in oral leukoplakia, oral submucous fibrosis, and healthy individuals: a comparative observational study

Akshaya Thiruvalluvan; Jagat R.C. Reddy; Vandana Sekizhar; Veni Subramanyam; Sivasankari T.; Varsha V.

doi:10.5114/jos.2021.111644

eISSN: 2299-551X
ISSN: 0011-4553

Journal of Stomatology

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abstract:

Original paper

Estimation of salivary matrix metalloproteinase-9 in oral leukoplakia, oral submucous fibrosis, and healthy individuals: a comparative observational study

Akshaya Thiruvalluvan

¹

,

Jagat R.C. Reddy

¹

,

Vandana Sekizhar

¹

,

Veni Subramanyam

²

,

Sivasankari T.

¹

,

Varsha V.

²

Department of Oral Medicine and Radiology, Indira Gandhi Institute of Dental Sciences, Sri Balaji Vidyapeeth (Deemed to be university), Puducherry, India
Central Inter-Disciplinary Research Facility (CIDRF), Sri Balaji Vidyapeeth, Pillaiyarkuppam, Puducherry, India

J Stoma 2021; 74, 4: 221-226

DOI: https://doi.org/10.5114/jos.2021.111644

Online publish date: 2021/12/19

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AMA

Thiruvalluvan A, R.C. Reddy J, Sekizhar V, Subramanyam V, T. S, V. V. Estimation of salivary matrix metalloproteinase-9 in oral leukoplakia, oral submucous fibrosis, and healthy individuals: a comparative observational study. Journal of Stomatology. 2021;74(4):221-226. doi:10.5114/jos.2021.111644.

APA

Thiruvalluvan, A., R.C. Reddy, J., Sekizhar, V., Subramanyam, V., T., S.,  & V., V. (2021). Estimation of salivary matrix metalloproteinase-9 in oral leukoplakia, oral submucous fibrosis, and healthy individuals: a comparative observational study. Journal of Stomatology, 74(4), 221-226. https://doi.org/10.5114/jos.2021.111644

Chicago

Thiruvalluvan, Akshaya, Jagat R.C. Reddy, Vandana Sekizhar, Veni Subramanyam, Sivasankari T.,  and Varsha V. 2021. "Estimation of salivary matrix metalloproteinase-9 in oral leukoplakia, oral submucous fibrosis, and healthy individuals: a comparative observational study". Journal of Stomatology 74 (4): 221-226. doi:10.5114/jos.2021.111644.

Harvard

Thiruvalluvan, A., R.C. Reddy, J., Sekizhar, V., Subramanyam, V., T., S.,  and V., V. (2021). Estimation of salivary matrix metalloproteinase-9 in oral leukoplakia, oral submucous fibrosis, and healthy individuals: a comparative observational study. Journal of Stomatology, 74(4), pp.221-226. https://doi.org/10.5114/jos.2021.111644

MLA

Thiruvalluvan, Akshaya et al. "Estimation of salivary matrix metalloproteinase-9 in oral leukoplakia, oral submucous fibrosis, and healthy individuals: a comparative observational study." Journal of Stomatology, vol. 74, no. 4, 2021, pp. 221-226. doi:10.5114/jos.2021.111644.

Vancouver

Thiruvalluvan A, R.C. Reddy J, Sekizhar V, Subramanyam V, T. S, V. V. Estimation of salivary matrix metalloproteinase-9 in oral leukoplakia, oral submucous fibrosis, and healthy individuals: a comparative observational study. Journal of Stomatology. 2021;74(4):221-226. doi:10.5114/jos.2021.111644.

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Introduction
Salivary matrix metalloproteinase-9 (MMP-9) is an established diagnostic biomarker for detection and prognosis of oral cancer.

Objectives: The current study aimed to estimate salivary MMP-9 in oral leukoplakia and oral submucous fibrosis (OSMF), which are considered as oral potentially malignant disorders (OPMDs).

Material and methods
A comparative observational study of 60 subjects was conducted, including 20 patients diagnosed with OSMF (group A, n = 20), 20 diagnosed with oral leukoplakia (group B, n = 20), and 20 as healthy controls (group C, n = 20) according to standard criteria. Unstimulated salivary samples were collected for evaluation of MMP-9 by enzyme-linked immunosorbent assay (ELISA). One-way analysis of variance (ANOVA) test was applied for comparison of groups. Bonferroni-adjusted one-way ANOVA test was performed for intra-group comparison.

Results
Descriptive statistics of the data revealed that mean age of study participants in group A, group B, and group C were 50.30 ± 9.96 years, 46.70 ± 12.59 years, and 33.30 ± 4.70 years, respectively. Mean salivary MMP-9 level in OSMF, oral leukoplakia, and control group were 9.42 ng/ml, 10.59 ng/ml, and 2.96 ng/ml, respectively, with a p-value < 0.001 between OSMF and oral leukoplakia when compared with controls.

Conclusions
A significant difference was noted in salivary MMP-9 levels among patients with OPMD’s compared to controls. A slightly higher value of MMP-9 was observed in oral leukoplakia. Estimation of salivary MMP-9 level is a non-invasive and a reliable biomarker in diagnosis of OPMD’s and in accessing their malignant transformation.

keywords:

Estimation of salivary matrix metalloproteinase-9 in oral leukoplakia, oral submucous fibrosis, and healthy individuals: a comparative observational study

Akshaya Thiruvalluvan 1 , Jagat R.C. Reddy 1 , Vandana Sekizhar 1 , Veni Subramanyam 2 , Sivasankari T. 1 , Varsha V. 2

matrix metalloproteinase-9, oral potentially malignant disorders, oral leukoplakia, oral submucous fibrosis, oral squamous cell carcinoma

Akshaya Thiruvalluvan

¹

,

Jagat R.C. Reddy

¹

,

Vandana Sekizhar

¹

,

Veni Subramanyam

²

,

Sivasankari T.

¹

,

Varsha V.

²