Evaluation of cytokine gene expression in psoriasis

Dakshinamurthy Divyapriya; Munisamy Priyadarssini; Sundar Indhumathi; Medha Rajappa; Laxmisha Chandrasekhar; Palani Selvam Mohanraj

doi:10.5114/ada.2021.110109

eISSN: 2299-0046
ISSN: 1642-395X

Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii

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abstract:

Original paper

Evaluation of cytokine gene expression in psoriasis

Dakshinamurthy Divyapriya

¹

,

Munisamy Priyadarssini

¹

,

Sundar Indhumathi

¹

,

Medha Rajappa

¹

,

Laxmisha Chandrasekhar

²

,

Palani Selvam Mohanraj

¹

Department of Biochemistry, Jawaharlal Institute of Post Graduate Medical Education and Research, Puducherry, India
Department of Dermatology, Jawaharlal Institute of Post Graduate Medical Education and Research, Puducherry, India

Adv Dermatol Allergol 2021; XXXVIII (5): 858–865

DOI: https://doi.org/10.5114/ada.2021.110109

Online publish date: 2021/11/05

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Introduction
Psoriasis is a chronic inflammatory disease affecting the skin with an unclear etiological significance.

Aim: In this study, we determined the mRNA expression and circulating levels of T helper (Th)/T regulatory (Treg) cytokines in psoriasis and analyzed their association with disease severity and treatment response.

Material and methods
189 psoriasis patients and 189 controls were recruited. Circulating Th/Treg cytokines (IL-12, IFN-g, IL-17, IL-23, TGF-b and IL-4) were measured at baseline and at follow-up after 12 weeks of methotrexate treatment by ELISA and their relative mRNA expression at baseline was estimated by quantitative PCR.

Results
We observed increased levels of Th1/Th17 cytokines (IFN-g, IL-17, IL-12 and IL-23) and a decrease in levels of Th2/Treg cytokines (IL-4 and TGF-b) in psoriasis patients at baseline, as compared to controls. Further, we observed that there was a significant decrease in Th1/Th17 cytokines, whilst Th2/Treg cytokine levels were significantly increased on follow-up after treatment with systemic metho trexate, as compared to pre-treatment levels. Our results were further confirmed by the significantly higher mRNA expression of Th1/Th17 cytokine genes and significantly lower mRNA expression of Th2/Treg cytokine genes in patients with psoriasis, as compared to controls. A significant positive correlation of Th1/Th17 cytokines was observed with disease severity in cases, whilst Th2/Treg cytokines correlated negatively with disease severity.

Conclusions
Our results show that increased Th1/Th17 cytokines and decreased Th2/Treg cytokines, both at the circulatory and gene expression level, play an important role in the immunopathogenesis and severity of psoriasis.

keywords:

Evaluation of cytokine gene expression in psoriasis

Dakshinamurthy Divyapriya 1 , Munisamy Priyadarssini 1 , Sundar Indhumathi 1 , Medha Rajappa 1 , Laxmisha Chandrasekhar 2 , Palani Selvam Mohanraj 1

cytokines, interleukins, gene expression, psoriasis

Dakshinamurthy Divyapriya

¹

,

Munisamy Priyadarssini

¹

,

Sundar Indhumathi

¹

,

Medha Rajappa

¹

,

Laxmisha Chandrasekhar

²

,

Palani Selvam Mohanraj

¹