Evaluation of morphology and angiogenesis of breast cancer in BALB/c mice using trypsin inhibitor from Cucumis melo seeds. In vitro and in vivo study

Shahla Rezaei; Negar Azarpira; Farhad Koohpeyma; Reza Yousefi; Saeid Doaei; Mojdeh Heidari; Maryam Gholamalizadeh; Mohammad Haghshenas; Zohreh Mazloom

doi:10.5114/wo.2022.120700

eISSN: 1897-4309
ISSN: 1428-2526

Contemporary Oncology/Współczesna Onkologia

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abstract:

Original paper

Evaluation of morphology and angiogenesis of breast cancer in BALB/c mice using trypsin inhibitor from Cucumis melo seeds. In vitro and in vivo study

Shahla Rezaei

¹

,

Negar Azarpira

²

,

Farhad Koohpeyma

³

,

Reza Yousefi

⁴

,

Saeid Doaei

⁵

,

Mojdeh Heidari

²

,

Maryam Gholamalizadeh

⁶

,

Mohammad Reza Haghshenas

⁷

,

Zohreh Mazloom

⁸

Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Shiraz Endocrinology and Metabolism Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Protein Chemistry Laboratory, Department of Biology, College of Sciences, Shiraz University, Shiraz, Iran
Department of Community Nutrition, National Nutrition and Food Technology Research Institute, Faculty of Nutrition Sciences and Food Technology, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Cancer Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Shiraz Institute for Cancer Research, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Clinical Nutrition, School of Nutrition and Food Sciences, Shiraz University of Medical Sciences, Shiraz, Iran

Contemp Oncol (Pozn) 2022; 26 (3): 204–219

DOI: https://doi.org/10.5114/wo.2022.120700

Online publish date: 2022/10/24

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AMA

Rezaei S, Azarpira N, Koohpeyma F, et al. Evaluation of morphology and angiogenesis of breast cancer in BALB/c mice using trypsin inhibitor from Cucumis melo seeds. In vitro and in vivo study. Contemporary Oncology/Współczesna Onkologia. 2022;26(3):204-219. doi:10.5114/wo.2022.120700.

APA

Rezaei, S., Azarpira, N., Koohpeyma, F., Yousefi, R., Doaei, S.,  & Heidari, M. et al. (2022). Evaluation of morphology and angiogenesis of breast cancer in BALB/c mice using trypsin inhibitor from Cucumis melo seeds. In vitro and in vivo study. Contemporary Oncology/Współczesna Onkologia, 26(3), 204-219. https://doi.org/10.5114/wo.2022.120700

Chicago

Rezaei, Shahla, Negar Azarpira, Farhad Koohpeyma, Reza Yousefi, Saeid Doaei, Mojdeh Heidari,  and Maryam Gholamalizadeh et al. 2022. "Evaluation of morphology and angiogenesis of breast cancer in BALB/c mice using trypsin inhibitor from Cucumis melo seeds. In vitro and in vivo study". Contemporary Oncology/Współczesna Onkologia 26 (3): 204-219. doi:10.5114/wo.2022.120700.

Harvard

Rezaei, S., Azarpira, N., Koohpeyma, F., Yousefi, R., Doaei, S., Heidari, M., Gholamalizadeh, M., Haghshenas, M.,  and Mazloom, Z. (2022). Evaluation of morphology and angiogenesis of breast cancer in BALB/c mice using trypsin inhibitor from Cucumis melo seeds. In vitro and in vivo study. Contemporary Oncology/Współczesna Onkologia, 26(3), pp.204-219. https://doi.org/10.5114/wo.2022.120700

MLA

Rezaei, Shahla et al. "Evaluation of morphology and angiogenesis of breast cancer in BALB/c mice using trypsin inhibitor from Cucumis melo seeds. In vitro and in vivo study." Contemporary Oncology/Współczesna Onkologia, vol. 26, no. 3, 2022, pp. 204-219. doi:10.5114/wo.2022.120700.

Vancouver

Rezaei S, Azarpira N, Koohpeyma F, Yousefi R, Doaei S, Heidari M et al. Evaluation of morphology and angiogenesis of breast cancer in BALB/c mice using trypsin inhibitor from Cucumis melo seeds. In vitro and in vivo study. Contemporary Oncology/Współczesna Onkologia. 2022;26(3):204-219. doi:10.5114/wo.2022.120700.

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Introduction
Melon seeds, as an excellent source of protease inhibitors, may have a protective role against tumor progression and angiogenesis. However, their effects on angiogenesis and the mechanism of their action against cancer progression remain elusive. This study aimed to investigate the effect of bioactive compounds of melon seed on the expression of angiogenesis genes in BALB/c mice with breast cancer.

Material and methods
Trypsin inhibitor (TI) was purified from the seed powder of Cucumis melo. Half- maximal inhibitory concentration was determined for TI, extract of melon seed powder (EXT), and tamoxifen (TAM) by the (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Also, breast tumor was induced by subcutaneous injection of MC4–L2 cells in BALB/c inbred mice breast tissue. After tumor growth, mice were treated with TI, EXT, and TAM to examine their effects on the tumor characteristics and expression of angiogenesis-related genes including MMP-2, MMP-9, and vascular endothelial growth factor (VEGF) using the reverse transcription polymerase chain reaction method.

Results
Trypsin inhibitor, EXT, TAM, and adjuvant treatment of TI + TAM resulted a reduction in expression of MMP-2, MMP-9, and VEGF. All treatments improved the breast tumor characteristics and the necrosis. The real time polymerase chain reaction method verified the positive effects of the treatments on the breast cancer cell line and tumors.

Conclusions
The results indicated that treatments with TI purified from Cucumis melo seeds and also combination therapy of TI and TAM can be considered as an alternative therapy in breast cancer patients. Further studies are warranted.

keywords:

Evaluation of morphology and angiogenesis of breast cancer in BALB/c mice using trypsin inhibitor from Cucumis melo seeds. In vitro and in vivo study

Shahla Rezaei 1 , Negar Azarpira 2 , Farhad Koohpeyma 3 , Reza Yousefi 4 , Saeid Doaei 5 , Mojdeh Heidari 2 , Maryam Gholamalizadeh 6 , Mohammad Reza Haghshenas 7 , Zohreh Mazloom 8

breast cancer, trypsin inhibitor, angiogenesis, melon seed extract

Shahla Rezaei

¹

,

Negar Azarpira

²

,

Farhad Koohpeyma

³

,

Reza Yousefi

⁴

,

Saeid Doaei

⁵

,

Mojdeh Heidari

²

,

Maryam Gholamalizadeh

⁶

,

Mohammad Reza Haghshenas

⁷

,

Zohreh Mazloom

⁸