Evaluation of spatial PD1 and PD-L1 expression in inflammatory bowel disease samples – a pilot study

Kinga Szczepaniak; Wiktor Paskal; Zofia Kuśmierczyk; Jarosław Wejman; Joanna Ostrowska; Wiesław Tarnowski; Paweł Włodarski

doi:10.5114/pjp.2022.117178

eISSN: 2084-9869
ISSN: 1233-9687

Polish Journal of Pathology

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1/2022
vol. 73

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abstract:

Original paper

Evaluation of spatial PD1 and PD-L1 expression in inflammatory bowel disease samples – a pilot study

Kinga Szczepaniak

¹

,

Wiktor Paskal

¹

,

Zofia Kuśmierczyk

¹

,

Jarosław Wejman

²

,

Joanna Ostrowska

²

,

Wiesław Tarnowski

³

,

Paweł Włodarski

¹

Department of Methodology, Medical University of Warsaw, Warsaw, Poland
Department of Pathology, Professor Witold Orlowski Public Clinical Hospital, Medical Center of Postgraduate Education, Warsaw, Poland
Department of Surgery, Professor Witold Orlowski Public Clinical Hospital, Medical Center of Postgraduate Education, Warsaw, Poland

Pol J Pathol 2022; 74 (1): 50-59

DOI: https://doi.org/10.5114/pjp.2022.117178

Online publish date: 2022/06/29

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Alterations of PD1/PD-L1 pathway may be associated with an excessive inflammatory response in the intestinal wall in inflammatory bowel diseases (IBD). To evaluate the expression of PD-1 and PD-L1 in 4 compartments of intestinal wall (mucosa, submucosa, muscularis propria and lymphatic follicles), high-resolution immunohistochemically stained slides were obtained from formalin-fixed paraffin-embedded samples of 10 Crohn’s disease (CD), 9 ulcerative colitis (UC) and 10 unaffected individuals cases. The levels of expression were quantified using the QuPath software.

PD-1 was detected in lymphatic follicles in affected and unaffected tissue samples and in inflammatory infiltration in IBD. There was no difference between groups neither in PD-1 overall expression nor in individual compartments, with the exception of the mucosal expression. It was higher in the mucosa of CD patients comparing to controls, however this difference was marginal (p = 0.0461). PD-L1 was expressed in endothelium and mesenteric nervous plexi, consistently in each group. There were no significant differences in PD-L1 immunoreactivity in context of histologic compartment nor clinical diagnosis. The results suggest that PD-1 and PD-L1 expression in intestinal tissue is heterogeneous in the analysed groups, thus it may be dependent on individual characteristics.

keywords:

Evaluation of spatial PD1 and PD-L1 expression in inflammatory bowel disease samples – a pilot study

Kinga Szczepaniak 1 , Wiktor Paskal 1 , Zofia Kuśmierczyk 1 , Jarosław Wejman 2 , Joanna Ostrowska 2 , Wiesław Tarnowski 3 , Paweł Włodarski 1

PD1, PDL1, IBD, ulcerative colitis, Crohn’s disease

Kinga Szczepaniak

¹

,

Wiktor Paskal

¹

,

Zofia Kuśmierczyk

¹

,

Jarosław Wejman

²

,

Joanna Ostrowska

²

,

Wiesław Tarnowski

³

,

Paweł Włodarski

¹