Experimental immunology<br>An anti-DEC-205 monoclonal antibody stimulates binding of thymocytes to rat thymic dendritic cells and promotes apoptosis of thymocytes

Ivana Majstorović; Dragana Vučević; Bojan Pavlović; Saša Vasilijić; Miodrag Čolić

doi:10.5114/ceji.2014.47722

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures

Editorial System

Submit your Manuscript

4/2014
vol. 39

Send email

Copy url:

abstract:

Experimental immunology
An anti-DEC-205 monoclonal antibody stimulates binding of thymocytes to rat thymic dendritic cells and promotes apoptosis of thymocytes

Ivana Majstorović

,

Dragana Vučević

,

Bojan Pavlović

,

Saša Vasilijić

,

Miodrag Čolić

(Centr Eur J Immunol 2014; 39 (4): 411-418)

DOI: https://doi.org/10.5114/ceji.2014.47722

Online publish date: 2014/12/15

View full text Get citation

EndNote

Papers, Reference Manager, RefWorks, Zotero

AMA

Majstorović I, Vučević D, Pavlović B, Vasilijić S, Čolić M. Experimental immunologyAn anti-DEC-205 monoclonal antibody stimulates binding of thymocytes to rat thymic dendritic cells and promotes apoptosis of thymocytes. Central European Journal of Immunology. 2014;39(4):411-418. doi:10.5114/ceji.2014.47722.

APA

Majstorović, I., Vučević, D., Pavlović, B., Vasilijić, S.,  & Čolić, M. (2014). Experimental immunologyAn anti-DEC-205 monoclonal antibody stimulates binding of thymocytes to rat thymic dendritic cells and promotes apoptosis of thymocytes. Central European Journal of Immunology, 39(4), 411-418. https://doi.org/10.5114/ceji.2014.47722

Chicago

Majstorović, Ivana, Dragana Vučević, Bojan Pavlović, Saša Vasilijić,  and Miodrag Čolić. 2014. "Experimental immunologyAn anti-DEC-205 monoclonal antibody stimulates binding of thymocytes to rat thymic dendritic cells and promotes apoptosis of thymocytes". Central European Journal of Immunology 39 (4): 411-418. doi:10.5114/ceji.2014.47722.

Harvard

Majstorović, I., Vučević, D., Pavlović, B., Vasilijić, S.,  and Čolić, M. (2014). Experimental immunologyAn anti-DEC-205 monoclonal antibody stimulates binding of thymocytes to rat thymic dendritic cells and promotes apoptosis of thymocytes. Central European Journal of Immunology, 39(4), pp.411-418. https://doi.org/10.5114/ceji.2014.47722

MLA

Majstorović, Ivana et al. "Experimental immunologyAn anti-DEC-205 monoclonal antibody stimulates binding of thymocytes to rat thymic dendritic cells and promotes apoptosis of thymocytes." Central European Journal of Immunology, vol. 39, no. 4, 2014, pp. 411-418. doi:10.5114/ceji.2014.47722.

Vancouver

Majstorović I, Vučević D, Pavlović B, Vasilijić S, Čolić M. Experimental immunologyAn anti-DEC-205 monoclonal antibody stimulates binding of thymocytes to rat thymic dendritic cells and promotes apoptosis of thymocytes. Central European Journal of Immunology. 2014;39(4):411-418. doi:10.5114/ceji.2014.47722.

PlumX metrics:

DEC-205, a transmembrane receptor responsible for cross-presentation of apoptotic cell-derived antigens, is expressed by cortical thymic epithelial cells (TEC) and thymic dendritic cells (TDC) in humans and mice, but its function in T-cell development is still unclear. In this work we have studied for the first time the expression of DEC-205 in the rat thymus by HD83 monoclonal antibody (mAb) and immunohistochemistry, as well as the ability of this mAb to modulate thymocyte – TDC interactions in vitro. We showed the positivity of cortical TEC in situ, including thymic nurse cells (TNC) in suspension, and TDC, whereas subcapsular, perivascular and medullary TEC were negative. All examined DEC-205 positive and DEC-205 negative structures were MHC class II positive. HD83 mAb increased apoptosis of thymocytes in co-culture with TDC in vitro and the process was associated with increased binding of thymocytes to TDC in a rosette form. Since negative selection of thymocytes by clonal deletion (apoptosis) was mediated predominantly by TDC, our results suggest the possible indirect effect of the DEC-205 molecule in these mechanisms.

keywords:

Experimental immunologyAn anti-DEC-205 monoclonal antibody stimulates binding of thymocytes to rat thymic dendritic cells and promotes apoptosis of thymocytes

Ivana Majstorović , Dragana Vučević , Bojan Pavlović , Saša Vasilijić , Miodrag Čolić

rat thymus, TDC, DEC-205, thymocyte apoptosis

Experimental immunology
An anti-DEC-205 monoclonal antibody stimulates binding of thymocytes to rat thymic dendritic cells and promotes apoptosis of thymocytes

Ivana Majstorović

,

Dragana Vučević

,

Bojan Pavlović

,

Saša Vasilijić

,

Miodrag Čolić