eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
4/2010
vol. 35
 
Share:
Share:
abstract:

Experimental immunology
Detection of antigenic variation among strains of poultry mycoplasma by immunoblot analysis

Gintautas Vyšniauskis
,
Irena Dumalakiené
,
Irena Jonauskiené

(Centr Eur J Immunol 2010; 35 (4): 207-213)
Online publish date: 2011/01/13
View full text Get citation
 
Immunoblotting analysis revealed immunodominant properties and the most intensive cross-reactivity of 39-44 kDa, 64-70 kDa and 77-80 kDa proteins of mycoplasma strains and isolates tested. Non-pathogenic M. gallinarum species isolates did not express antigenic heterogeneity. Protein polymorphism of pathogenic M. synoviae species isolates were determined by both the differences between intensity of expression of 77-88 kDa protein and the differences in size of 38-46 kDa proteins. Soluble antigen fraction and polyvalent sera testing by immunoblotting analysis enables to determine morphological or antigenic heterogeneity of strains but fails to reveal species-specific features.
keywords:

poultry mycoplasma, immunobot analysis, polyclonal antisera

Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.