eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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4/2010
vol. 35
 
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abstract:

Experimental immunology
Detection of antigenic variation among strains of poultry mycoplasma by immunoblot analysis

Gintautas Vyšniauskis
,
Irena Dumalakiené
,
Irena Jonauskiené

(Centr Eur J Immunol 2010; 35 (4): 207-213)
Online publish date: 2011/01/13
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Immunoblotting analysis revealed immunodominant properties and the most intensive cross-reactivity of 39-44 kDa, 64-70 kDa and 77-80 kDa proteins of mycoplasma strains and isolates tested. Non-pathogenic M. gallinarum species isolates did not express antigenic heterogeneity. Protein polymorphism of pathogenic M. synoviae species isolates were determined by both the differences between intensity of expression of 77-88 kDa protein and the differences in size of 38-46 kDa proteins. Soluble antigen fraction and polyvalent sera testing by immunoblotting analysis enables to determine morphological or antigenic heterogeneity of strains but fails to reveal species-specific features.
keywords:

poultry mycoplasma, immunobot analysis, polyclonal antisera
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