Experimental immunology<br>HSV-2-infected monocytes and keratinocytes show different inflammatory reaction in response to Fas receptor stimulation

Małgorzata Krzyżowska; Anna Winnicka; Wanda Stankiewicz

doi:10.5114/ceji.2013.35219

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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2/2013
vol. 38

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abstract:

Experimental immunology
HSV-2-infected monocytes and keratinocytes show different inflammatory reaction in response to Fas receptor stimulation

Małgorzata Krzyżowska

,

Anna Winnicka

,

Wanda Stankiewicz

(Centr Eur J Immunol 2013; 38 (2): 196-202)

DOI: https://doi.org/10.5114/ceji.2013.35219

Online publish date: 2013/07/08

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In the present paper we assessed the role of Fas in the expression of pro-inflammatory cytokines and chemokines during HSV-2 infection using keratinocyte and monocyte cells in vitro. While uninfected keratinocyte and monocyte cultures responded with apoptosis induction upon Fas receptor stimulation with cytotoxic antibody, HSV-2-infected monocytes, but not keratinocytes, were susceptible to Fas-induced apoptosis. Both uninfected, Fas-stimulated and HSV-2-infected keratinocytes and only infected monocytes up-regulated the expression of pro-inflammatory chemokines – chemokine (C-X-C motif) ligand 1 (CXCL1) and CXCL10 and cytokines – interferon α (IFN-α) and tumour necrosis factor α (TNF-α). HSV-2 infection of monocytes and keratinocytes led to up-regulation of TNF-α, IFN-α, CXCL1 and CXCL10 expression. Stimulation of Fas receptor reduced the expression of tested cytokines and chemokines in HSV-2-infected keratinocyte cultures, but not in monocytes. Furthermore, while apoptosis blocking down-regulated the expression of IFN-α, CXCL1 and CXCL10 in HSV-2-infected monocytes, the keratinocytes showed the opposite effect for IFN-α, and CXCL10. We conclude that the Fas/FasL pathway participates in controlling the pro-inflammatory response during HSV-2 infection, albeit its effect depends on the type of infected cells.

keywords:

Experimental immunologyHSV-2-infected monocytes and keratinocytes show different inflammatory reaction in response to Fas receptor stimulation

Małgorzata Krzyżowska , Anna Winnicka , Wanda Stankiewicz

HSV-2, apoptosis, Fas, keratinocyte, monocyte

Experimental immunology
HSV-2-infected monocytes and keratinocytes show different inflammatory reaction in response to Fas receptor stimulation

Małgorzata Krzyżowska

,

Anna Winnicka

,

Wanda Stankiewicz