Experimental immunology
In vitro assessment of the proliferative abilities of lymphocytes treated with Respisure vaccine as well as immunomodulators Lydium-KLP and Methisoprinol

There are no reports in the literature on the effect of vaccine antigen supplemented with immunomodulators on the activity of T and B lymphocytes. The aim of this study was to determine the proliferative activity of lymphocytes treated with a vaccine against mycoplasmal pneumonia of swine (MPS) and with selected immunomodulators in in vitro examinations. Five clinically healthy piglets at the age of 21 days were used in the experiment and blood for lymphocyte isolation was collected from them. Inactivated vaccine against MPS – Respisure (Pfizer) – at dilutions of 1 : 10, 1 : 100 and 1 : 1000 and immunomodulators – Methisoprinol (Polfa Grodzisk, Poland) and Lydium- KLP (Nika Health Products Ltd, USA), were also used. The test of mitogen-stimulated lymphocyte proliferation was performed according to the MTT method. T lymphocytes were stimulated with ConA, whereas B lymphocytes were stimulated with LPS. The experiment showed that combinations of immunomodulators: Lydium-KLP and Methisoprinol and the Respisure vaccine at dilutions of 1 : 100 and 1 : 1000 increase in vitro proliferative activity of ConA-stimulated T lymphocytes and LPS-stimulated B lymphocytes. A stronger proliferative response of both the lymphocyte types was observed for the combination of the Respisure vaccine and Methisoprinol.