Experimental immunology
Influence of Lactobacillus rhamnosus GG metabolites on immunological processes in vitro

The Lactobacillus species belong to bacteria favourably modulating human immune response, nevertheless they may cause infection in extreme immunodeficiency disorders. Several cases of infections provoked by Lactobacillus strains referred to: short intestine syndrome, prematurity and status after cardiosurgery operation. The aim of this paper was to evaluate the influence of Lactobacillus rhamnosus GG (L.GG) metabolites on certain immunity components in vitro. The bacteria were placed inside alginate microcapsules to prevent host invasion by L.GG.

No growth of L.GG was observed on the external surface of membranes under scanning microscope examination, proving the microcapsules were impenetrable for bacteria. Further the microcapsules containing L.GG were lyophilised and added to a culture of PHA stimulated human lymphocytes obtained from the whole blood. Statistically significant increase of lymphoblasts percentage was noted. While the medium significant increase of IL-5 concentration and only a slight increase of TNF-β concentration were observed