eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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3/2014
vol. 39
 
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abstract:

Experimental immunology
Xenotransplantation of human cultured parathyroid progenitor cells into mouse peritoneum does not induce rejection reaction

Ireneusz Nawrot
,
Bogdan Woźniewicz
,
Jacek Szmidt
,
Dariusz Śladowski
,
Krzysztof Zając
,
Witold Chudziński

(Centr Eur J Immunol 2014; 39 (3): 279-284)
DOI: https://doi.org/10.5114/ceji.2014.45937
Online publish date: 2014/10/14
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Introduction: Parathyroid progenitor cells devoid of immunogenic antigens were used for human allotransplantation. Although there were many potential reasons for the expiry of transplant activity in humans, we decided to exclude a subclinical form of rejection reaction, and test the rejection reaction in an animal model.

Material and methods: Experiments were carried out on 40 conventional male mice in their third month of life. The animals were housed in groups of 10 per cage in 4 cages with fitted water dispensers and fed a conventional diet based on standard pellet food. They were divided into four groups of 10 animals each, three experimental groups and one control group. Identified progenitor cells were stored in a cell bank. After testing the phenotype, viability, and absence of immunogenic properties, the cells were transplanted into mouse peritoneum cavity.

Results: Animals were observed for 9 weeks. At 9 weeks of observation, the mean serum PTH concentration in the experimental groups was 2.0-2.5 pg/ml, while in the control group it did not exceed 1.5 pg/ml. The immunohistochemical assays demonstrated that millions of viable cells with a phenotype identical to the endocrine cells had survived in the peritoneum. Histologic specimens from different internal organs stained for PTH revealed positive cells labelled with anti-PTH Ab in the intestinal lamina, brain, liver, and spleen.

Conclusions: In the present paper we have demonstrated that xenotransplantation may be used as a model for an explanation of the immunogenic properties of cells generated from postnatal organs for regenerative therapy.
keywords:

parathyroid progenitor cells, xenotransplantation, rejection
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