Expression of ORAI1 and STIM1 genes in blood
of patients with pulmonary tuberculosis

Oksana Kolesova; Ksenija Kramica; Aleksandrs Kolesovs; Jelena Eglite

doi:10.5114/ceji.2021.106998

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures

Editorial System

Submit your Manuscript

2/2021
vol. 46

Send email

Copy url:

abstract:

Short communication

Expression of ORAI1 and STIM1 genes in blood of patients with pulmonary tuberculosis

Oksana Kolesova

¹

,

Ksenija Kramica

¹

,

Aleksandrs Kolesovs

¹

,

Jelena Eglite

¹

Riga Stradins University, Latvia

Cent Eur J Immunol 2021; 46 (2): 275-282

DOI: https://doi.org/10.5114/ceji.2021.106998

Online publish date: 2021/06/18

View full text Get citation

PlumX metrics:

This study aimed to detect the expression level of ORAI1 and STIM1 genes in blood of patients with bilateral pulmonary tuberculosis (TB) in comparison with the control group. Both genes encode proteins providing store-operated Ca2+ entry (SOCE) into the cells, including immune cells, to activate transcriptional factors for producing cytokines and inflammation-restricting proteins. The study included 45 patients with confirmed TB, aged 20 to 86, and 35 volunteers, aged from 21 to 73, without active TB infection. The expression of ORAI1 and STIM1 genes in blood was performed by real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the referent gene. Inflammation was assessed by levels of interferon  (IFN-) and interleukin 18 (IL-18) in serum (ELISA method). The results showed lower expression of ORAI1 in blood and higher levels of IFN- and IL-18 in serum of TB patients than that of the control group and no differences in expression of the STIM1 gene. It indicates some impairment in the SOCE mechanism of immune cells, which is associated with TB.

keywords:

Expression of ORAI1 and STIM1 genes in blood of patients with pulmonary tuberculosis

Oksana Kolesova 1 , Ksenija Kramica 1 , Aleksandrs Kolesovs 1 , Jelena Eglite 1

cytokines, tuberculosis, SOCE, mRNA ORAI1, mRNA STIM1

Oksana Kolesova

¹

,

Ksenija Kramica

¹

,

Aleksandrs Kolesovs

¹

,

Jelena Eglite

¹