Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer

Aldona Kasprzak; Witold Szaflarski; Jacek Szmeja; Małgorzata Andrzejewska; Wiesława Przybyszewska; Maria Koczorowska; Michał Drews; Elżbieta Kaczmarek

doi:10.5114/wo.2012.28794

eISSN: 1897-4309
ISSN: 1428-2526

Contemporary Oncology/Współczesna Onkologia

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2/2012
vol. 16

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abstract:

Original paper

Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer

Aldona Kasprzak

,

Witold Szaflarski

,

Jacek Szmeja

,

Małgorzata Andrzejewska

,

Wiesława Przybyszewska

,

Maria Koczorowska

,

Michał Drews

,

Elżbieta Kaczmarek

Wspolczesna Onkol 2012; 16 (2): 147–153

DOI: https://doi.org/10.5114/wo.2012.28794

Online publish date: 2012/05/29

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Aim of the study: Several epidemiological studies have attempted to demonstrate a relationship between increased serum level of insulin-like growth factor 1 (IGF-1) and an augmented risk of developing colorectal cancers (CRC). The human IGF-1 gene is composed of 6 exons and demonstrated expression of 6 different splice variants (isoforms) of mRNA (IA, IB, IC, IIA, IIB and IIC).The aim of the study was to evaluate the expression of different isoforms of IGF-1 mRNA in CRC and normal colon tissue.

Material and methods: 13 paired tissue specimens (colorectal tumor and non-tumor tissues) were analyzed using both quantitative polymerase chain reaction (PCR) and immunocytochemistry methods (IHC). The expression of classes I and II and variants A, B, C of IGF-1 mRNA were measured.

Results: In CRC higher amounts of IGF-1 class II mRNA than class I mRNA were detected. Among A, B, C isoforms, A variant of IGF-1 mRNA prevailed. The amounts of IGF-1 class I and class II mRNAs and of IGF-1 variant B mRNA were lowered in CRC as compared to the control. In CRC significant correlations were detected between reciprocal expression of class I and class II as well as between I and II isoforms and A, B and C.

Conclusions: Expression of IGF-1 mRNA isoforms differs between normal and CRC tissues. Even if all isoforms of IGF-1 mRNA manifested correlations with each other in tissues of CRC, expression of all transcripts (except that of isoform A) was significantly decreased as compared to the control.

keywords:

Expression of various insulin-like growth factor-1 mRNA isoforms in colorectal cancer

Aldona Kasprzak , Witold Szaflarski , Jacek Szmeja , Małgorzata Andrzejewska , Wiesława Przybyszewska , Maria Koczorowska , Michał Drews , Elżbieta Kaczmarek

colorectal carcinoma, IGF-1 isoforms, QPCR, immunocytochemistry

Aldona Kasprzak

,

Witold Szaflarski

,

Jacek Szmeja

,

Małgorzata Andrzejewska

,

Wiesława Przybyszewska

,

Maria Koczorowska

,

Michał Drews

,

Elżbieta Kaczmarek