First successful DNA isolation and profiling from bone using an approach that is non-destructive toward bone surface

Dominika Pluta; Arleta Lebioda; Anna Jonkisz; Tadeusz Dobosz

doi:10.5114/amsik.2016.62335

eISSN: 1689-1716
ISSN: 0324-8267

Archiwum Medycyny Sądowej i Kryminologii/Archives of Forensic Medicine and Criminology

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1/2016
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abstract:

Letter to the Editor

First successful DNA isolation and profiling from bone using an approach that is non-destructive toward bone surface

Dominika Pluta

¹

,

Arleta Lebioda

¹

,

Anna Jonkisz

¹

,

Tadeusz Dobosz

¹

Department of Molecular Techniques, Chair of Forensic Medicine, Wroclaw Medical University, Wroclaw, Poland

Arch Med Sąd Kryminol 2016; 66 (1): 65–70

DOI: https://doi.org/10.5114/amsik.2016.62335

Online publish date: 2016/09/16

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AMA

Pluta D, Lebioda A, Jonkisz A, Dobosz T. First successful DNA isolation and profiling from bone using an approach that is non-destructive toward bone surface. Archiwum Medycyny Sądowej i  Kryminologii/Archives of Forensic Medicine and Criminology. 2016:65-70. doi:10.5114/amsik.2016.62335.

APA

Pluta, D., Lebioda, A., Jonkisz, A.,  & Dobosz, T. (2016). First successful DNA isolation and profiling from bone using an approach that is non-destructive toward bone surface. Archiwum Medycyny Sądowej i  Kryminologii/Archives of Forensic Medicine and Criminology, 65-70. https://doi.org/10.5114/amsik.2016.62335

Chicago

Pluta, Dominika, Arleta Lebioda, Anna Jonkisz,  and Tadeusz Dobosz. 2016. "First successful DNA isolation and profiling from bone using an approach that is non-destructive toward bone surface". Archiwum Medycyny Sądowej i  Kryminologii/Archives of Forensic Medicine and Criminology: 65-70. doi:10.5114/amsik.2016.62335.

Harvard

Pluta, D., Lebioda, A., Jonkisz, A.,  and Dobosz, T. (2016). First successful DNA isolation and profiling from bone using an approach that is non-destructive toward bone surface. Archiwum Medycyny Sądowej i  Kryminologii/Archives of Forensic Medicine and Criminology, pp.65-70. https://doi.org/10.5114/amsik.2016.62335

MLA

Pluta, Dominika et al. "First successful DNA isolation and profiling from bone using an approach that is non-destructive toward bone surface." Archiwum Medycyny Sądowej i  Kryminologii/Archives of Forensic Medicine and Criminology, 2016, pp. 65-70. doi:10.5114/amsik.2016.62335.

Vancouver

Pluta D, Lebioda A, Jonkisz A, Dobosz T. First successful DNA isolation and profiling from bone using an approach that is non-destructive toward bone surface. Archiwum Medycyny Sądowej i  Kryminologii/Archives of Forensic Medicine and Criminology. 2016:65-70. doi:10.5114/amsik.2016.62335.

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Commonly used destructive DNA isolation techniques consist of pulverization which leads to the complete destruction of smaller bones or irreversible damage to larger bones through the cutting of extensive fragments. The procedure is totally unacceptable when handling bones which are valuable for anthropological or religious reasons, as museum exhibits or due to emotional factors. Since the Department’s team has already resolved this problem in the case of human teeth (A. Pilecka), efforts have been undertaken to develop a similar non-destructive technique for the isolation of DNA from human bones. To the best knowledge of the authors of the report, the study has yielded the world’s first STR profile derived from DNA isolated from human bones by a technique that is non-destructive toward the bone surface.

keywords:

First successful DNA isolation and profiling from bone using an approach that is non-destructive toward bone surface

Dominika Pluta 1 , Arleta Lebioda 1 , Anna Jonkisz 1 , Tadeusz Dobosz 1

bones, DNA isolation, nondestructive method

Dominika Pluta

¹

,

Arleta Lebioda

¹

,

Anna Jonkisz

¹

,

Tadeusz Dobosz

¹