eISSN: 1689-1716
ISSN: 0324-8267
Archiwum Medycyny Sądowej i Kryminologii/Archives of Forensic Medicine and Criminology
Current issue Archive Manuscripts accepted About the journal Supplements Editorial board Reviewers Abstracting and indexing Subscription Contact Instructions for authors Ethical standards and procedures
SCImago Journal & Country Rank
1/2016
vol. 66
 
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abstract:
Letter to the Editor

First successful DNA isolation and profiling from bone using an approach that is non-destructive toward bone surface

Dominika Pluta
1
,
Arleta Lebioda
1
,
Anna Jonkisz
1
,
Tadeusz Dobosz
1

  1. Department of Molecular Techniques, Chair of Forensic Medicine, Wroclaw Medical University, Wroclaw, Poland
Arch Med Sąd Kryminol 2016; 66 (1): 65–70
Online publish date: 2016/09/16
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Commonly used destructive DNA isolation techniques consist of pulverization which leads to the complete destruction of smaller bones or irreversible damage to larger bones through the cutting of extensive fragments. The procedure is totally unacceptable when handling bones which are valuable for anthropological or religious reasons, as museum exhibits or due to emotional factors. Since the Department’s team has already resolved this problem in the case of human teeth (A. Pilecka), efforts have been undertaken to develop a similar non-destructive technique for the isolation of DNA from human bones. To the best knowledge of the authors of the report, the study has yielded the world’s first STR profile derived from DNA isolated from human bones by a technique that is non-destructive toward the bone surface.
keywords:

bones, DNA isolation, nondestructive method

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