Generation of high cross-presentation ability human dendritic cells by combination
of interleukin 4, interferon β and GM-CSF

Yihua Pi; Yifang Li; Rongyi Liang; Jian Xiao; Jing Leng; Lifeng Zhang

doi:10.5114/ceji.2022.117767

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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2/2022
vol. 47

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abstract:

Experimental immunology

Generation of high cross-presentation ability human dendritic cells by combination of interleukin 4, interferon β and GM-CSF

Yihua Pi

¹

,

Yifang Li

²

,

Rongyi Liang

^{1, 3}

,

Jian Xiao

^{1, 3}

,

Jing Leng

^{1, 3}

,

Lifeng Zhang

^{1, 3}

Guangxi University of Chinese Medicine, China
The Second People’s Hospital of Hunan Province, China
Guangxi Key Laboratory of Translational Medicine for Treating High-Incidence Infectious Diseases with Integrative Medicine, China

Cent Eur J Immunol 2022; 47 (2): 125-138

DOI: https://doi.org/10.5114/ceji.2022.117767

Online publish date: 2022/07/04

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Dendritic cell (DC)-based immunotherapies have been utilized for the treatment of numerous diseases. However, the conventional generation strategies of DCs in vitro require 7 days and these DCs showed an unsatisfactory function, which prompted us to explore new approaches. We found that in vitro culture of human CD14+ cells, in the medium containing granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin (IL)-4, as well as interferon  (IFN-) for 48 h, followed by the maturation stimuli of IL-1 and poly I:C for another 24 h can be differentiated into high cross-presentation ability DCs (G4B-DCs). These DCs express high levels of CD11c, CD86, and HLA-DR, producing a high level of tumor necrosis factor  (TNF-). Of note, compared with the conventional DCs, G4B-DCs showed a higher ability to promote allogeneic naïve CD4+ T cell and CD8+ T cell proliferation and interferon (IFN)- production. These DCs also have the remarkable ability to induce Flu-M1-specific CD8+ T cells. In addition, we found that these G4B-DCs express partially the cDC1 phenotype. These data indicate that G4B-DC is unique and may provide a relatively rapid alternative method for potential clinical use.

keywords:

Generation of high cross-presentation ability human dendritic cells by combination of interleukin 4, interferon β and GM-CSF

Yihua Pi 1 , Yifang Li 2 , Rongyi Liang 1, 3 , Jian Xiao 1, 3 , Jing Leng 1, 3 , Lifeng Zhang 1, 3

IL-4, dendritic cell, cDC1, cross-presentation, IFN-b

Yihua Pi

¹

,

Yifang Li

²

,

Rongyi Liang

^{1, 3}

,

Jian Xiao

^{1, 3}

,

Jing Leng

^{1, 3}

,

Lifeng Zhang

^{1, 3}