Current issue
Archive
Videos
Articles in press
About the journal
Supplements
Editorial board
Reviewers
Abstracting and indexing
Subscription
Contact
Instructions for authors
Publication charge
Ethical standards and procedures
Editorial System
Submit your Manuscript
|
2/2005
vol. 107 abstract:
Original paper
HLA-DR antygen expression on conjunctival epithelial cells in patients with dry eye
Małgorzata Mrugacz
1
,
Nella Żywalewska
1
Klinika Oczna 2005, 107(4-6): 278-279
Online publish date: 2005/07/02
View
full text
Get citation
ENW EndNote
BIB JabRef, Mendeley
RIS Papers, Reference Manager, RefWorks, Zotero
AMA
APA
Chicago
Harvard
MLA
Vancouver
Introduction
The aim of this study was to investigate the expression of HLA-DR antigen on conjunctival epithelial cells from patients with dry eye. Material and methods Ophthalmic examination and dry eye diagnostic tests were performed. Impression cytology specimens were collected in thirty patients with dry eye and in thirty people of control group. Conjunctival epithelial cells were processed for flow cytometry, by using monoclonal antibodies to HLA-DR. Results A significant increase of HLA-DR expression on epithelial cells was found in patients with dry eye compared with normal eyes. We also found positive correlation of increased expression HLA-DR with ocular complaints and diagnostic tests for dry eye such as Schirmer test, FBUT and lissamine green staining. Conclusions These results confirm the presence of immunological processes in the pathogenesis of dry eye syndrome. Increased expression of HLA-DR antygen positively correlates with the parameters of dry eye diagnostic tests. HLA-DR measurement might be a useful method for monitoring inflammatory conjunctival changes in this disorder. keywords:
inflammation, flow cytometry, impression cytology, dry eye |
|