Haploidentical allogeneic haematopoietic cell transplantation using CD3/CD19 depletion and myeloablative conditioning: a case report and a review of the literature

Jan Styczyński; Małgorzata Rochnowska; Robert Dębski; Elżbieta Hulek; Anna Krenska; Hanna Górnicka; Paweł Wojtylak; Mariusz Wysocki

eISSN: 1897-4309
ISSN: 1428-2526

Contemporary Oncology/Współczesna Onkologia

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6/2009
vol. 13

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abstract:

Case report

Haploidentical allogeneic haematopoietic cell transplantation using CD3/CD19 depletion and myeloablative conditioning: a case report and a review of the literature

Jan Styczyński

,

Małgorzata Rochnowska

,

Robert Dębski

,

Elżbieta Hulek

,

Anna Krenska

,

Hanna Górnicka

,

Paweł Wojtylak

,

Mariusz Wysocki

Współczesna Onkologia (2009) vol. 13; 6 (292–296)

Online publish date: 2010/01/04

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AMA

Styczyński J, Rochnowska M, Dębski R, et al. Haploidentical allogeneic haematopoietic cell transplantation using CD3/CD19 depletion and myeloablative conditioning: a case report and a review of the literature. Contemporary Oncology/Współczesna Onkologia. 2009;13(6):292-296.

APA

Styczyński, J., Rochnowska, M., Dębski, R., Hulek, E., Krenska, A.,  & Górnicka, H. et al. (2009). Haploidentical allogeneic haematopoietic cell transplantation using CD3/CD19 depletion and myeloablative conditioning: a case report and a review of the literature. Contemporary Oncology/Współczesna Onkologia, 13(6), 292-296.

Chicago

Styczyński, Jan, Małgorzata Rochnowska, Robert Dębski, Elżbieta Hulek, Anna Krenska, Hanna Górnicka,  and Paweł Wojtylak et al. 2009. "Haploidentical allogeneic haematopoietic cell transplantation using CD3/CD19 depletion and myeloablative conditioning: a case report and a review of the literature". Contemporary Oncology/Współczesna Onkologia 13 (6): 292-296.

Harvard

Styczyński, J., Rochnowska, M., Dębski, R., Hulek, E., Krenska, A., Górnicka, H., Wojtylak, P.,  and Wysocki, M. (2009). Haploidentical allogeneic haematopoietic cell transplantation using CD3/CD19 depletion and myeloablative conditioning: a case report and a review of the literature. Contemporary Oncology/Współczesna Onkologia, 13(6), pp.292-296.

MLA

Styczyński, Jan et al. "Haploidentical allogeneic haematopoietic cell transplantation using CD3/CD19 depletion and myeloablative conditioning: a case report and a review of the literature." Contemporary Oncology/Współczesna Onkologia, vol. 13, no. 6, 2009, pp. 292-296.

Vancouver

Styczyński J, Rochnowska M, Dębski R, Hulek E, Krenska A, Górnicka H et al. Haploidentical allogeneic haematopoietic cell transplantation using CD3/CD19 depletion and myeloablative conditioning: a case report and a review of the literature. Contemporary Oncology/Współczesna Onkologia. 2009;13(6):292-296.

A haploidentical transplant can be defined as a transplant that uses marrow or stem cells from a relative who is only half matched in HLA. Recently, a new regimen using graft CD3/CD19 depletion with anti-CD3- and anti-CD19-coated microbeads on a CliniMACS device was developed. CD3/CD19 depleted grafts contain not only CD34+ stem cells but also CD34-negative progenitors, natural killer, dendritic and graft-facilitating cells, thereby enhancing engraftment. We report a case description of a child using CD3/CD19 depleted grafts in a haploidentical stem cell transplant setting and a review of the available literature. A 3-year old boy was treated for very early relapse of acute lymphoblastic leukaemia. The myelo-ablative therapy consisted of weight-adjusted intravenous busulfan, flu-darabine, cyclophosphamide, and thymoglobuline. The reduction of CD3 load was 99.9% (i.e. 3.64 log scale); however, due to current CD3 load in the graft (2.7 × 105 CD3+/kg), GVHD prophylaxis was done with cyclosporine A and methotrexate. The only complication after transplantation was CMV reactivation, treated with pre-emptive therapy. Bone marrow biopsy at day +29 revealed normal haematopoiesis and donor chimerism 95%. The patient remains in remission at day +300. We can conclude from our observation and literature review that HLA haploidentical peripheral blood stem cell transplantation with CD3/CD19 depletion may be an effective and safe therapy for children with relapsed leukaemia.

keywords:

Haploidentical allogeneic haematopoietic cell transplantation using CD3/CD19 depletion and myeloablative conditioning: a case report and a review of the literature

Jan Styczyński , Małgorzata Rochnowska , Robert Dębski , Elżbieta Hulek , Anna Krenska , Hanna Górnicka , Paweł Wojtylak , Mariusz Wysocki

stem cell transplantation, children, CD3/CD19 depletion, acute lymphoblastic leukaemia

Jan Styczyński

,

Małgorzata Rochnowska

,

Robert Dębski

,

Elżbieta Hulek

,

Anna Krenska

,

Hanna Górnicka

,

Paweł Wojtylak

,

Mariusz Wysocki