How fast does wasp venom immunotherapy affect a regulatory T cell subpopulation (CD4+ CD25+ Foxp3+) and the synthesis of interleukins 10, 21 and transforming growth factor β1?

Aleksander Zakrzewski; Jerzy Kruszewski; Andrzej Chciałowski; Krzysztof Kłos; Agnieszka Rzeszotarska; Jolanta Korsak; Ewa M. Nowosielska; Aneta Cheda; Jolanta Wrembel-Wargocka; Marek K. Janiak

doi:10.5114/ada.2019.82828

eISSN: 2299-0046
ISSN: 1642-395X

Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii

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abstract:

Original paper

How fast does wasp venom immunotherapy affect a regulatory T cell subpopulation (CD4+ CD25+ Foxp3+) and the synthesis of interleukins 10, 21 and transforming growth factor β1?

Aleksander Zakrzewski

,

Jerzy Kruszewski

,

Andrzej Chciałowski

,

Krzysztof Kłos

,

Agnieszka Rzeszotarska

,

Jolanta Korsak

,

Ewa M. Nowosielska

,

Aneta Cheda

,

Jolanta Wrembel-Wargocka

,

Marek K. Janiak

Adv Dermatol Allergol 2019; XXXVI (1): 82-85

DOI: https://doi.org/10.5114/ada.2019.82828

Online publish date: 2019/02/22

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AMA

Zakrzewski A, Kruszewski J, Chciałowski A, et al. How fast does wasp venom immunotherapy affect a regulatory T cell subpopulation (CD4+ CD25+ Foxp3+) and the synthesis of interleukins 10, 21 and transforming growth factor β1?. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2019;36(1):82-85. doi:10.5114/ada.2019.82828.

APA

Zakrzewski, A., Kruszewski, J., Chciałowski, A., Kłos, K., Rzeszotarska, A.,  & Korsak, J. et al. (2019). How fast does wasp venom immunotherapy affect a regulatory T cell subpopulation (CD4+ CD25+ Foxp3+) and the synthesis of interleukins 10, 21 and transforming growth factor β1?. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 36(1), 82-85. https://doi.org/10.5114/ada.2019.82828

Chicago

Zakrzewski, Aleksander, Jerzy Kruszewski, Andrzej Chciałowski, Krzysztof Kłos, Agnieszka Rzeszotarska, Jolanta Korsak,  and Ewa M. Nowosielska et al. 2019. "How fast does wasp venom immunotherapy affect a regulatory T cell subpopulation (CD4+ CD25+ Foxp3+) and the synthesis of interleukins 10, 21 and transforming growth factor β1?". Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii 36 (1): 82-85. doi:10.5114/ada.2019.82828.

Harvard

Zakrzewski, A., Kruszewski, J., Chciałowski, A., Kłos, K., Rzeszotarska, A., Korsak, J., Nowosielska, E., Cheda, A., Wrembel-Wargocka, J.,  and Janiak, M. (2019). How fast does wasp venom immunotherapy affect a regulatory T cell subpopulation (CD4+ CD25+ Foxp3+) and the synthesis of interleukins 10, 21 and transforming growth factor β1?. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 36(1), pp.82-85. https://doi.org/10.5114/ada.2019.82828

MLA

Zakrzewski, Aleksander et al. "How fast does wasp venom immunotherapy affect a regulatory T cell subpopulation (CD4+ CD25+ Foxp3+) and the synthesis of interleukins 10, 21 and transforming growth factor β1?." Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, vol. 36, no. 1, 2019, pp. 82-85. doi:10.5114/ada.2019.82828.

Vancouver

Zakrzewski A, Kruszewski J, Chciałowski A, Kłos K, Rzeszotarska A, Korsak J et al. How fast does wasp venom immunotherapy affect a regulatory T cell subpopulation (CD4+ CD25+ Foxp3+) and the synthesis of interleukins 10, 21 and transforming growth factor β1?. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2019;36(1):82-85. doi:10.5114/ada.2019.82828.

Introduction
The literature describes the influence of venom immunotherapy (VIT) on the subpopulation of T regulatory cells (CD4+ CD25+ Foxp3+) and the synthesis of IL-10, TGF-1 as well as many other cytokines at various times after immunotherapy.

Aim
To assess changes in the percentage of cells of CD4+ and CD25+ in peripheral blood and serum concentrations of IL-10, IL-21 and TGF-1 in the early stages of VIT.

Material and methods
The study included 18 patients who were allergic to wasp venom and who in the past underwent systemic anaphylactic reaction after stinging, meeting the criteria to qualify for VIT. The immunoenzymatic method (ELISA) was used to assess concentrations of cytokines IL-10, IL-21 and TGF-1 and the surface antigens CD4 and CD25 on the cells. The concentrations were determined by flow cytometry method at baseline (before VIT) and after 2.5 and 24 h from the VIT starting point.

Results
The mean values of the activity of T lymphocytes CD4+ CD25+ FoxP3+ and concentrations of the cytokines IL-10, IL-21 and TGF-1 are shown in table.

Conclusions
A 24-hour activation assessment of serum concentrations of cytokines IL-10, IL-21 and TGF-1 during the first day of the Hymenoptera venom immunotherapy by ultra-rush protocol does not show the significant dynamics of change of the examined parameters.

keywords:

How fast does wasp venom immunotherapy affect a regulatory T cell subpopulation (CD4+ CD25+ Foxp3+) and the synthesis of interleukins 10, 21 and transforming growth factor β1?

Aleksander Zakrzewski , Jerzy Kruszewski , Andrzej Chciałowski , Krzysztof Kłos , Agnieszka Rzeszotarska , Jolanta Korsak , Ewa M. Nowosielska , Aneta Cheda , Jolanta Wrembel-Wargocka , Marek K. Janiak

cytokines, regulatory cells, Hymenoptera venom immunotherapy

Aleksander Zakrzewski

,

Jerzy Kruszewski

,

Andrzej Chciałowski

,

Krzysztof Kłos

,

Agnieszka Rzeszotarska

,

Jolanta Korsak

,

Ewa M. Nowosielska

,

Aneta Cheda

,

Jolanta Wrembel-Wargocka

,

Marek K. Janiak