eISSN: 2354-0265
ISSN: 2353-6942
Health Problems of Civilization Physical activity: diseases and issues recognized by the WHO
Current issue Archive Online first About the journal Editorial board Reviewers Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
3/2017
vol. 11
 
Share:
Share:
OTHER
abstract:
Review paper

IMPROVED RAT STROKE MODEL BY INTRALUMINAL MIDDLE CEREBRAL ARTERY OCCLUSION: A SPECIAL EMPHASIS ON SURGICAL TECHNIQUE

Alex Orădan
1
,
Adina Huțanu
2, 3
,
Emőke Horváth
4
,
Liviu Chiriac
5
,
Minodora Dobreanu
2, 3

  1. Laboratory animal facility – Centre for Experimental Medicine, Iuliu Hațieganu University of Medicine and Pharmacy, Cluj-Napoca, Romania
  2. Center for Advanced Medical and Pharmaceutical Research, University of Medicine and Pharmacy of Târgu-Mureș, Romania
  3. Department of Laboratory Medicine, University of Medicine and Pharmacy of Târgu-Mureș, Romania
  4. Department of Pathology, University of Medicine and Pharmacy, Târgu-Mureş, Romania
  5. National Magnetic Resonance Center, Faculty of Physics, Babes-Bolyai University, Cluj-Napoca, Romania
Health Problems of Civilization. 2017; 11(3): 202-210
Online publish date: 2017/10/26
View full text Get citation
 
PlumX metrics:
Various rodent models have been developed for examining either focal or global cerebral ischemia so far, but the most used model is the rat middle cerebral artery occlusion by intraluminal filaments, followed by reperfusion, and 2,3,5-triphenyltetrazoliumchloride or Cresyl Violet/Nissl staining for evaluation of the region of interest. Like other surgical procedures, this one is prone to various complications (excessive bleeding, vascular or nerve lesions) and failure related to the surgical technique. We focused on detailed surgical techniques, along with data from literature, in order to reduce the complications and increase the chance of experiment success. Magnetic Resonance Imaging evaluation with 3D reconstruction of the ischemic area opens new perspectives on investigations into the ischemic brain, having the advantage of being noninvasive as an alternative to gold histological standard for measuring the infarct size, being the only one accesible in surviving subjects.
keywords:

stroke, middle cerebral artery occlusion, cerebral ischemia


Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.