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1/2013
vol. 94 abstract:
Review paper
Identification of amino acid sequences via X-ray crystallography: a mini review of case studies
Agnieszka J. Pietrzyk
,
Anna Bujacz
,
Mariusz Jaskolski
,
Grzegorz Bujacz
BioTechnologia vol. 94(1) C pp. 9-14 C 2013
Online publish date: 2014/10/23
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The sequencing of a protein is a complicated issue. Several methods have been developed in order to establish
the amino acid sequences of proteins, including Edman degradation, LC/MS/MS and cDNA sequencing. A number of examples confirm that X-ray crystallography could also be a useful tool for the identification of an amino acid sequence of unknown proteins. Here, we present a short review summarizing the application of crystallography for protein sequence determination and identification. |