Identification of herpes simplex viruses in samples taken from ambulatory patients’ genital mucosa collected at The I Department of Gynecology & Obstetrics Medical University in Warsaw

Anna Majewska; Marek Kilijańczyk; Małgorzata Gajewska; Tomasz Dzieciątkowski; Mirosław Łuczak; Grażyna Młynarczyk

ISSN: 1231-6407

Ginekologia Praktyczna - - - ARCHIVAL

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4/2009
vol. 17

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abstract:

Identification of herpes simplex viruses in samples taken from ambulatory patients’ genital mucosa collected at The I Department of Gynecology & Obstetrics Medical University in Warsaw

Anna Majewska

,

Marek Kilijańczyk

,

Małgorzata Gajewska

,

Tomasz Dzieciątkowski

,

Mirosław Łuczak

,

Grażyna Młynarczyk

Gin Prakt 2009; 4: 17-20

Online publish date: 2009/12/30

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AMA

Majewska A, Kilijańczyk M, Gajewska M, Dzieciątkowski T, Łuczak M, Młynarczyk G. Identification of herpes simplex viruses in samples taken from ambulatory patients’ genital mucosa collected at The I Department of Gynecology & Obstetrics Medical University in Warsaw. Ginekologia Praktyczna  - - -       <span style="color: red">ARCHIVAL</span>. 2009:17-20.

APA

Majewska, A., Kilijańczyk, M., Gajewska, M., Dzieciątkowski, T., Łuczak, M.,  & Młynarczyk, G. (2009). Identification of herpes simplex viruses in samples taken from ambulatory patients’ genital mucosa collected at The I Department of Gynecology & Obstetrics Medical University in Warsaw. Ginekologia Praktyczna  - - -       <span style="color: red">ARCHIVAL</span>, 17-20.

Chicago

Majewska, Anna, Marek Kilijańczyk, Małgorzata Gajewska, Tomasz Dzieciątkowski, Mirosław Łuczak,  and Grażyna Młynarczyk. 2009. "Identification of herpes simplex viruses in samples taken from ambulatory patients’ genital mucosa collected at The I Department of Gynecology & Obstetrics Medical University in Warsaw". Ginekologia Praktyczna  - - -       <span style="color: red">ARCHIVAL</span>: 17-20.

Harvard

Majewska, A., Kilijańczyk, M., Gajewska, M., Dzieciątkowski, T., Łuczak, M.,  and Młynarczyk, G. (2009). Identification of herpes simplex viruses in samples taken from ambulatory patients’ genital mucosa collected at The I Department of Gynecology & Obstetrics Medical University in Warsaw. Ginekologia Praktyczna  - - -       <span style="color: red">ARCHIVAL</span>, pp.17-20.

MLA

Majewska, Anna et al. "Identification of herpes simplex viruses in samples taken from ambulatory patients’ genital mucosa collected at The I Department of Gynecology & Obstetrics Medical University in Warsaw." Ginekologia Praktyczna  - - -       <span style="color: red">ARCHIVAL</span>, 2009, pp. 17-20.

Vancouver

Majewska A, Kilijańczyk M, Gajewska M, Dzieciątkowski T, Łuczak M, Młynarczyk G. Identification of herpes simplex viruses in samples taken from ambulatory patients’ genital mucosa collected at The I Department of Gynecology & Obstetrics Medical University in Warsaw. Ginekologia Praktyczna  - - -       <span style="color: red">ARCHIVAL</span>. 2009:17-20.

Objectives: Identification of human herpesviruses type 1 and 2 in samples taken from female genital tract. Investigation of the clinical utility of various laboratory methods for the detection of genital herpes in woman.

Material and methods: Overall 66 genital swabs were collected from 46 women. All samples were tested using cell culture for the virus presence and for the HHV-1 and HHV-2 DNA presence using PCR after amplifi-cation of virus in cell culture (Vero cell line).

Results: Cytopathic effects in cell cultures were observed in 21 samples, viral DNA was detected in 20 samples. In our study we established high conformity a viral culture and PCR method (k = 0.803).

Conclusions: In this study viruses were detected in asymptomatic woman, probably because of subclinical infections in the sites, which are difficult to observe during routine gynecological examination. In this study HHV-1 was the main causative agent of genital herpes. HHV-1 is predominant causative agent of herpes oral cavity infection. The oral mucous membranes are probable a source of infection. Method - in witch virus was amplificated and than DNA typing using PCR - is particularly useful because enable to detect a type of virus. Now, it is very important, because of changing in the
epidemiology of HHV-1 and HHV-2 infections.

keywords:

Identification of herpes simplex viruses in samples taken from ambulatory patients’ genital mucosa collected at The I Department of Gynecology & Obstetrics Medical University in Warsaw

Anna Majewska , Marek Kilijańczyk , Małgorzata Gajewska , Tomasz Dzieciątkowski , Mirosław Łuczak , Grażyna Młynarczyk

genital herpes, micro-bial examination, polymerase chain reaction

Anna Majewska

,

Marek Kilijańczyk

,

Małgorzata Gajewska

,

Tomasz Dzieciątkowski

,

Mirosław Łuczak

,

Grażyna Młynarczyk