eISSN: 2084-9869
ISSN: 1233-9687
Polish Journal of Pathology
Current issue Archive Manuscripts accepted About the journal Supplements Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
1/2009
vol. 60
 
Share:
Share:
abstract:

Immunohistochemical pattern of protein P21, cyclin D1 and cyclin E in endometrial hyperplasia

Aleksandra Brucka
,
Piotr Bartczak
,
Marzena Ratyńska
,
Stanisław Sporny

Pol J Pathol 2009; 1: 19-25
Online publish date: 2009/05/05
View full text Get citation
 

Purpose: In our research we investigated immunohistochemical expression of cell cycle proteins protein p21, cyclin D1 and cyclin E in physiological endometrium (n = 15), hyperplastic endometrium (n = 61), and post hor-mone replacement therapy endometrium (n = 24).
Material and methods: We performed immunohistochemical analysis of selected cell cycle proteins in 100 specimens of human endometrium.
Results: The average immunoexpression index scores in glandular endometrial cells (GES) and stromal endometrial cells (SEC) were respectively: for p21- GES: 11.8 ±17.19%; SEC: 9.31 ±17.15%; for cyclin D1- GES: 9.25 ±18.41%; SEC: 3.22 ±11.46%; for cyclin E: GES: 26.42 ±27.47%; SEC: 4.61 ±7.90%. Statistical analysis disclosed more intense p21 glandular immunoreactivity among women with endometrial hyperplasia in comparison to other subpopulations. In the case of assessment of cyclin D1 immunoreactivity, there was no statistical correlation between analysed parameters. The average cyclin E immunoreactivity in endometrial glandular cells was significantly higher (p = 0.003) in women with endometrial hyperplasia and correlated with age.
Conclusions: Intensive immunoreactivity of cyclin E in glandular cells is typical for endometrial hyperplasia and can be treated as an objective indicator of this pathological process during histopathological diagnostic procedures. Immunoreactivity index of p21 and cyclin D1 is independent of the morphological pattern of human endometrium, patients’ age and gynaecological history of patients.
keywords:

carcinogenesis, cell cycle, endometrium, HRT, hyperplasia, neoplasia, regulatory proteins

Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.