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4/2013
vol. 94 abstract:
Short communication
Improvement of tannase production under submerged fermentation by Aspergillus niger FBT1 isolated from a mangrove forest
Dominic Soloman George
,
Chong-Boon Ong
BioTechnologia vol. 94(4) C pp. 451-456 C 2013
Online publish date: 2014/10/23
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Aspergillus niger FBT1, a local extracellular strain for tannase production, was isolated from soil collected from
Matang Mangrove Forest Reserve in Perak, Malaysia. This fungus strain was cultivated in an Erlenmeyer flask under a submerged fermentation system. Medium compositions play a very important role in enhancing enzyme production during fermentation. The production of tannase by A. niger FBT1 increased significantly (95%) when the medium compositions and various process parameters were optimized. Incubation for 72 hours (30EC, pH 7) in medium complemented with sodium nitrate was found optimal. Additional supplementation with tannic acid (2% w/v) as the sole carbon source strongly increased the yield of the enzyme. keywords:
tannase, submerged fermentation, Aspergillus niger, Mangrove |