Increased risks between TLR2 (-196 to -174 ins/del) and TLR3 1377C>T variants and head and neck cancers in Tunisia

Lamia Makni; Sabrina Zidi; Mouadh Barbirou; Amira Ben Ahmed; Ezzedine Gazouani; Amel Mezlini; Mouna Stayoussef; Besma Yacoubi-Loueslati

doi:10.5114/ceji.2019.87065

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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2/2019
vol. 44

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abstract:

Clinical immunology

Increased risks between TLR2 (-196 to -174 ins/del) and TLR3 1377C>T variants and head and neck cancers in Tunisia

Lamia Makni

¹

,

Sabrina Zidi

¹

,

Mouadh Barbirou

²

,

Amira Ben Ahmed

¹

,

Ezzedine Gazouani

²

,

Amel Mezlini

³

,

Mouna Stayoussef

¹

,

Besma Yacoubi-Loueslati

¹

Laboratory of Mycology, Pathologies, and Biomarkers: LR16ES05, Department of Biology, Faculty of Sciences, El Manar University, Tunis, Tunisia
Laboratory of Immunology, Military Hospital of Tunis, Tunis, Tunisia
Salah Azeiz Oncology Institute, Tunis, Tunisia

(Centr Eur J Immunol 2019; 44 (2): 144-149)

DOI: https://doi.org/10.5114/ceji.2019.87065

Online publish date: 2019/07/30

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Introduction
Previous studies have highlighted the importance of polymorphisms of toll-like receptors (TLRs) in the pathogenesis of certain cancers, including head and neck cancers (HNC).

Aim of the study
The aim of this study was to evaluate the association of TLR2 (-196 to -174 ins/del) and TLR3 (1377 C>T) as potential risk factors for HNC in Tunisians.

Material and methods
A case-control study including 246 HNC patients (174 nasopharyngeal carcinoma – NPC and 72 laryngeal cancer – LC) and 250 healthy controls. Genotyping was done by using PCR and PCR-RFLP methods.

Results
Higher minor allele frequencies of TLR2 (-196 to -174 ins/del) and TLR3 1377 C>T polymorphisms were seen in HNC, NPC, and LC compared to controls. In addition, higher increased HNC, NPC, and LC risk was associated with TLR2 ins/del and TLR2 del/del genotypes (p < 0.0001). Positive association with HNC, NPC, and LC risk was seen with TLR2 del-containing genotypes (ins/del + del/del) (p < 0.0001). The T/T genotype of TLR3 is associated with HNC, NPC, and LC susceptibility (p < 0.0001). Positive association with HNC and NPC risk was seen with TLR3 T allele carriers (C/T + T/T) (p < 0.0001). Increased frequency of T-ins, C-del, and T-del haplotypes was revealed in HNC and NPC cases than healthy controls; however, T-del was significantly higher in LC cases.

Conclusions
Our results demonstrate an increased risk of HNC, NPC, and LC with TLR2 ins/del, TLR2 del/del, and TLR3 T/T genotypes. And positive association with T-ins, C-del, and T-del haplotypes with HNC and NPC and T-del haplotype with LC.

keywords:

Increased risks between TLR2 (-196 to -174 ins/del) and TLR3 1377C>T variants and head and neck cancers in Tunisia

Lamia Makni 1 , Sabrina Zidi 1 , Mouadh Barbirou 2 , Amira Ben Ahmed 1 , Ezzedine Gazouani 2 , Amel Mezlini 3 , Mouna Stayoussef 1 , Besma Yacoubi-Loueslati 1

toll-like receptors, polymorphisms, laryngeal cancer, head and neck cancer, Tunisia, nasopharyngeal cancer

Lamia Makni

¹

,

Sabrina Zidi

¹

,

Mouadh Barbirou

²

,

Amira Ben Ahmed

¹

,

Ezzedine Gazouani

²

,

Amel Mezlini

³

,

Mouna Stayoussef

¹

,

Besma Yacoubi-Loueslati

¹